Attachment between heterotrophic bacteria and microalgae influences symbiotic microscale interactions

Environmental Microbiology
Ty J SamoXavier Mayali

Abstract

The surface and surroundings of microalgal cells (phycosphere) are critical interaction zones but have been difficult to functionally interrogate due to methodological limitations. We examined effects of phycosphere-associated bacteria for two biofuel-relevant microalgal species (Phaeodactylum tricornutum and Nannochloropsis salina) using stable isotope tracing and high spatial resolution mass spectrometry imaging (NanoSIMS) to quantify elemental exchanges at the single-cell level. Each algal species responded differently to bacterial attachment. In P. tricornutum, a high percentage of cells had attached bacteria (92%-98%, up to eight bacteria per alga) and fixed 64% more carbon with attached bacteria compared to axenic cells. In contrast, N. salina cells were less commonly associated with bacteria (42%-63%), harboured fewer bacteria per alga, and fixed 10% more carbon without attached bacteria compared to axenic cells. An uncultivated bacterium related to Haliscomenobacter sp. was identified as an effective mutualist; it increased carbon fixation when attached to P. tricornutum and incorporated 71% more algal-fixed carbon relative to other bacteria. Our results illustrate how phylogenetic identity and physical location of bact...Continue Reading

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Citations

Jul 13, 2019·Annual Review of Marine Science·Xavier Mayali
Jan 23, 2020·MBio·Sara L JackrelVincent J Denef
Sep 9, 2020·Environmental Microbiology·Cong FeiShady A Amin
Nov 28, 2020·Analytical and Bioanalytical Chemistry·Fangchen LiuMingming Wu
May 1, 2021·Biology·Lina Maria González-González, Luz E de-Bashan
May 21, 2021·PloS One·Hannah Laeverenz SchlogelhoferOttavio A Croze

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