PMID: 7543487May 1, 1995Paper

Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding

Journal of Virological Methods
T HankeR E Randall

Abstract

A small 14 amino acid oligopeptide tag (termed SV5-Pk) was fused onto the carboxy-terminus of simian immunodeficiency virus gp160 expressed from a recombinant baculovirus. The presence of the Pk tag had no obvious effect on the expression and glycosylation of gp160 and did not interfere either with CD4 binding or with cleavage at its maturation site by the protease furin. The presence of the Pk tag did, however, facilitate the simplified purification of full-length gp160 and its incorporation into immunogenic solid matrix-antibody-antigen (SMAA) complexes.

References

Mar 25, 1989·Nucleic Acids Research·C Livingstone, I Jones
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R L WilleyR D Klausner
Dec 1, 1993·AIDS Research and Human Retroviruses·J P Moore, Q J Sattentau
Mar 1, 1993·AIDS Research and Human Retroviruses·J B Jowett, I M Jones

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Citations

Jun 22, 2000·The Journal of General Virology·X LiJ W Lamb
Jan 12, 2021·Chembiochem : a European Journal of Chemical Biology·Philipp WolfOliver Seitz

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