PMID: 3771647Oct 1, 1986Paper

Attachment of cells to basement membrane collagen type IV

The Journal of Cell Biology
M Aumailley, R Timpl

Abstract

Of ten different cell lines examined, three showed distinct attachment and spreading on collagen IV substrates, and neither attachment nor spreading was enhanced by adding soluble laminin or fibronectin. This reaction was not inhibited by cycloheximide or antibodies to laminin, indicating a direct attachment to collagen IV without the need of mediator proteins. Cell-binding sites were localized to the major triple-helical domain of collagen IV and required an intact triple helical conformation for activity. Fibronectin showed preferential binding to denatured collagen IV necessary to mediate cell binding to the substrate. Fibronectin binding sites of collagen IV were mapped to unfolded structures of the major triple-helical domain and show a similar specificity to fibronectin-binding sites of collagen I. The data extend previous observations on biologically potential binding sites located in the triple helix of basement membrane collagen IV.

References

Apr 2, 1979·European Journal of Biochemistry·R TimplP Fietzek
Jan 1, 1979·The Journal of Cell Biology·J C MurrayS I Katz
Jun 1, 1978·The Journal of Experimental Medicine·E EngvallE J Miller
Mar 1, 1978·European Journal of Biochemistry·R TimplH Wiedemann
Jan 1, 1985·Journal of Cellular Biochemistry·H K KleinmanM DuBois-Dalcq
Jun 1, 1985·The Journal of Cell Biology·A S CharonisH Furthmayr
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Dec 27, 1983·Biochimica Et Biophysica Acta·D T WoodleyH K Kleinman
Aug 15, 1984·European Journal of Biochemistry·S FujiwaraJ Engel
Nov 1, 1981·European Journal of Biochemistry·R TimplK Kühn
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·V P TerranovaL A Liotta
Mar 29, 1983·Biochemical and Biophysical Research Communications·N C RaoL A Liotta
May 1, 1983·The Journal of Cell Biology·H L Malinoff, M S Wicha
Jan 1, 1983·Annual Review of Biochemistry·K M Yamada
Apr 10, 1984·Biochemistry·P D Yurchenco, H Furthmayr
Sep 1, 1981·Collagen and Related Research·J RisteliR Timpl
Mar 1, 1982·European Journal of Biochemistry·U OttR Timpl

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Citations

Jan 1, 1994·Journal of Cellular Physiology·D J DonaldsonA E Chung
Jan 1, 1992·Journal of Neuroscience Research·C MirreB Knibiehler
Mar 1, 1989·Clinical & Experimental Metastasis·L WeissK V Honn
Apr 30, 2010·Cellular and Molecular Life Sciences : CMLS·Jenny Kruegel, Nicolai Miosge
Oct 2, 2013·Indian Journal of Clinical Biochemistry : IJCB·Sandesh MohanJyoti Mannari
Oct 1, 1988·Developmental Biology·V Schmid, A Bally
Aug 1, 1990·Developmental Biology·M A HadleyM Dym
Jun 21, 1991·Brain Research. Developmental Brain Research·P J Lein, D Higgins
Feb 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·F RuggieroR Garrone
May 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·B KnibiehlerY Le Parco
Jan 1, 1995·Journal of Photochemistry and Photobiology. B, Biology·H U PüschelJ Brinckmann
Dec 1, 1987·Collagen and Related Research·M Paulsson
Sep 22, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·A G Marneros, B R Olsen
Mar 1, 1988·The Journal of Cell Biology·K R GehlsenE Ruoslahti
Mar 1, 1994·The Journal of Cell Biology·M L Matter, G W Laurie
Aug 5, 2000·Current Opinion in Nephrology and Hypertension·R Kalluri, V P Sukhatme
Jul 1, 1987·European Journal of Biochemistry·M PaulssonJ Engel
Apr 1, 1989·European Journal of Biochemistry·R Timpl
Sep 1, 1989·European Journal of Biochemistry·M AumailleyR Timpl
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·H HopferG Vollmer
May 1, 1991·American Journal of Respiratory Cell and Molecular Biology·K A RickardS I Rennard
Apr 1, 1989·Cell Biophysics·L Weiss, G W Schmid-Schönbein
Feb 11, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ahmed H MekkawyDavid L Morris
Jun 20, 2014·Molecular Biology of the Cell·Charles G MillerJean E Schwarzbauer
Jan 1, 1993·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·W M LesterM Tanaka
Jan 1, 1988·Scandinavian Journal of Gastroenterology. Supplement·U Hahn

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