PMID: 9544775Apr 17, 1998Paper

Attachment of group B streptococci to macrophages is mediated by a 21-kDa protein

FEMS Immunology and Medical Microbiology
L M SmithT G Pistole

Abstract

Group B Streptococcus (GBS) is able to bind to human macrophages in vitro in the absence of exogenous opsonins. The exact mechanisms that mediate this attachment are unclear. This study was undertaken to determine what protein adhesins are present on the surface of GBS that mediate attachment to macrophages. We have identified a 21-kDa protein from the envelope of GBS type III that directly binds to macrophages as determined by Western blot analysis. Antiserum against this protein was able to inhibit binding of GBS to macrophages by greater than 80% as measured by flow cytometry. Antiserum against the 21-kDa protein cross-reacted with 21-kDa proteins from GBS type Ib, type II, type III (COH31 and MR732) and type IV, as well as Staphyloccus epidermidis, but not GBS type Ia, Listeria monocytogenes or Enterococcus faecalis. This protein may be important in mediating the attachment of GBS to macrophages in an opsonin-poor environment.

References

May 15, 1976·International Journal of Cancer. Journal International Du Cancer·C Sundström, K Nilsson
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G RoveraC Damsky
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 1, 1992·Infection and Immunity·D L HastyR J Doyle
Jul 1, 1988·Reviews of Infectious Diseases·P Ferrieri
Feb 1, 1994·Infection and Immunity·J R Wang, M W Stinson
Jun 1, 1993·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·A R Sloan, T G Pistole
Mar 1, 1993·Applied and Environmental Microbiology·J A BradburneJ N Mathis
Jun 24, 1993·The New England Journal of Medicine·M M FarleyD S Stephens
Jun 24, 1993·The New England Journal of Medicine·M R Wessels, D L Kasper
Jul 1, 1996·Infection and Immunity·P Valentin-WeigandG S Chhatwal

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Citations

Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·G Malin, L C Paoletti
Jan 16, 2007·Journal of Microbiological Methods·Bochiwe Hara-Kaonga, Thomas G Pistole
Jan 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Soumita DasJames E Casanova
Jan 13, 2005·Canadian Journal of Microbiology·Bochiwe Hara-Kaonga, Thomas G Pistole

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