PMID: 39583Aug 1, 1979

Attachment of Neisseria gonorrhoeae to human sperm. Microscopical study of trypsin and iron

The British Journal of Venereal Diseases
C I GomezR P Williams

Abstract

Pilated Neisseria gonorrhoeae of colony type 1 (T1) and non-pilated bacteria of colony type 4 (T4) were observed by transmission (TEM) and scanning electron microscopy (SEM). No pili were observed on T4 gonogocci, but two types of pili--straight, type a, and bent, type b--were seen on T1 by TEM. When incubated with human sperum and examined by either TEM or SEM, T1 gonococci were seen to attach by individual pili, by several pili wound together as a rope, or by direct contact. Gonococci from T4 colonies attached only by direct contact. Treatment with typsin (1 mg/ml) damaged or removed pili from gonococci. After incubation with trypsin, attachment of pilated gonococci to sperm was decreased significantly, but such treatment did not affect attachment of non-pilated gonococci. Incubation of gonococci from either colony type in 0.1 mmol/l ferric nitrate, followed by incubation with sperm, significantly increased attachment of only T4 bacteria. No pili were seen on T4 gonococci treated with ferric nitrate; thus, it appears that factors other than pili alone are concerned in attachment of these gonococci to sperm.

References

Dec 1, 1996·Urology·R K Sharma, A Agarwal
May 15, 1984·American Journal of Obstetrics and Gynecology·L G KeithJ Friberg
Jan 1, 1991·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·W A Bonner
Feb 1, 1996·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·W A Bonner
Jan 5, 2018·Antonie van Leeuwenhoek·Elise S PelzerFlavia Huygens
Jan 4, 2001·Clinical and Diagnostic Laboratory Immunology·F GorgaE Galdiero
Jul 11, 2019·Scientific Reports·Andrew D WintersKevin R Theis
Jul 22, 2020·Andrologia·Ralf HenkelDavid Fisher

Citations

Jul 1, 1975·Journal of General Microbiology·P Novotny, W H Turner
Apr 1, 1976·The British Journal of Venereal Diseases·A N JamesR P Williams
Aug 1, 1977·Journal of Medical Microbiology·P NovotnyI P MacLennan
Sep 1, 1977·Journal of General Microbiology·J N RobertsonM E Ward
Jun 1, 1975·The Journal of Experimental Medicine·T M Buchanan
Aug 1, 1975·Journal of Medical Microbiology·P NovotnyP D Walker
Dec 1, 1975·Infection and Immunity·S M Payne, R A Finkelstein
Oct 1, 1976·Journal of General Microbiology·J E HeckelsM E Ward
Oct 1, 1971·The Journal of Experimental Medicine·J SwansonE C Gotschlich
Oct 1, 1974·The British Journal of Venereal Diseases·A Grimble, L R Armitage
Aug 1, 1973·Infection and Immunity·A P Punsalang, W D Sawyer
Nov 1, 1973·The Journal of Clinical Investigation·T M BuchananE C Gotschlich
May 1, 1974·Infection and Immunity·A N James-HolmquestR P Williams
Jan 1, 1968·Annual Review of Microbiology·R G Bartsch
Jan 1, 1969·Annual Review of Medicine·D S Kellogg, J D Thayer
Jul 1, 1971·The Journal of Urology·T L Howard
Feb 1, 1971·Journal of General Microbiology·G A Wistreich, R F Baker

Related Concepts

Lysogeny
Ferric Compounds
Alzheimer Disease 4
Electron Microscopy
Phage Attachment Sites
Y-Chromosome-Bearing Sperm
Bacterial Pilus
Neisseria gonorrhoeae
Trypure
Scanning Electron Microscopy

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.