PMID: 9181551Apr 1, 1997Paper

Attachment of osteoblastic cells to hydroxyapatite crystals by a synthetic peptide (Glu7-Pro-Arg-Gly-Asp-Thr) containing two functional sequences of bone sialoprotein

Matrix Biology : Journal of the International Society for Matrix Biology
R FujisawaY Kuboki

Abstract

We investigated activity of bone sialoprotein (BSP) to mediate attachment of cells to hydroxyapatite using a model peptide, Glu7-Pro-Arg-Gly-Asp-Thr, which contains a putative hydroxyapatite-binding site (poly-Glu) and a cell-attachment site. The peptide has affinity to hydroxyapatite with a dissociation constant of 13.5 microM. The peptide affected in vitro mineralization in a gel system, indicating interaction between this peptide and calcium phosphate. The osteoblastic cell line MC3T3-E1 was incubated with hydroxyapatite powder coated with the peptide or proteins. Attachment of the cells was observed on the powder coated with BSP, but not on the powder coated with serum albumin. The cells were attached to the powder coated with the peptide. The cells were flattened on the powder, and pseudopods developed. The attachment of the cells was inhibited by an excessive amount of Gly-Arg-Gly-Asp-Ser peptide. In conclusion, BSP mediated attachment of osteoblastic cells to hydroxyapatite, and this activity could be accomplished only by the poly-Glu sequence and the Arg-Gly-Asp sequence.

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Citations

Sep 28, 2010·Biotechnology Letters·Dan WangJing-Tao Zhang
Nov 22, 2007·Journal of Materials Science. Materials in Medicine·Vivian WangBrian Amsden
Jan 22, 2010·Journal of Materials Science. Materials in Medicine·Yan LiuShiqiang Gong
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Apr 4, 2021·Journal of Functional Biomaterials·George BullockCheryl Miller
Apr 29, 2008·Biomaterials·Kristin M HennessySusan L Bellis

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