PMID: 6164986Mar 11, 1981Paper

Attachment of protein affinity-labeling reagents of variable length and amino acid specificity to E. coli tRNAfMet

Nucleic Acids Research
L H SchulmanS A Reines

Abstract

Transamination with bifunctional amines in the presence of bisulfite has been used to attach side chains of variable length to the N4-position of single stranded cytidine residues in E. coli tRNAfMet. Such side chains, terminating in reactive primary amino groups, have been coupled to a variety of N-hydroxysuccinimide esters. The resulting modified tRNAs carry protein affinity labeling groups capable of covalent reaction with a variety of amino acids.

References

Apr 13, 1979·Biochemical and Biophysical Research Communications·P M AbdellaG P Royer
Jul 11, 1973·Journal of the American Chemical Society·M SonoH Hayatsu
Feb 6, 1974·Journal of the American Chemical Society·R ShapiroM Welcher
Feb 11, 1970·Journal of the American Chemical Society·H HayatsuK Kazushige
Aug 24, 1970·Biochemical and Biophysical Research Communications·R Shapiro, J M Weisgras

❮ Previous
Next ❯

Citations

Apr 1, 1992·Genetic Analysis, Techniques and Applications·E DaviniP Zappelli
Feb 12, 2008·Journal of Neuroscience Methods·Dannelys Pérez-BelloChryslaine Rodríguez-Tanty
Jul 1, 1989·Journal of Bioluminescence and Chemiluminescence·M Septak
Mar 15, 1984·Biochemical and Biophysical Research Communications·D ValenzuelaL H Schulman
Feb 12, 2005·Preparative Biochemistry & Biotechnology·David Higginson ClarkeEsteban Gutiérrez Calzado
Jan 1, 1992·Bioconjugate Chemistry·P S Miller, C D Cushman
Aug 25, 1987·Biochemistry·O Leon, L H Schulman
Feb 1, 1986·Journal of Clinical Microbiology·R P ViscidiR H Yolken
Jul 16, 1990·Biochemical and Biophysical Research Communications·C AvignoloF A Bignone

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.