Attempt to discriminate between bovine viral-diarrhoea virus strains using polymerase chain reaction

Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B
M TajimaK Takahashi

Abstract

The polymerase chain reaction (PCR) was applied to detect bovine viral-diarrhoea mucosal-disease virus (BVDV). By the use of properly prepared primers, cytopathogenic NADL, Oregon C24V, Nose, T-20 and KS86-1(+) strains, and non-cytopathogenic New York-1, No. 12, and KS86-1(-) strains could be detected. The PCR system was applied to field isolates of the viruses. All the viruses were detected by the PCR. Four patterns of the PCR amplification were recognized, and it was possible to discriminate between some strains. These results corresponded with the serotype of BVDV, as determined by the serum-neutralizing test. The BVDV gene was detectable from the leucocytes of infected cattle using the PCR method. Moreover, it was possible to detect and discriminate BVDV strains using one PCR tube that included all primer pairs.

References

Oct 1, 1992·Zentralblatt für Veterinärmedizin. Reihe A·M TajimaK Takahashi
Jan 1, 1991·Veterinary Microbiology·C HertigE Peterhans
Sep 1, 1991·Journal of Virology·H J ThielG Meyers
Jan 1, 1990·Archives of Virology·B A Schroeder, T C Balassu-Chan
Jan 1, 1989·Veterinary Microbiology·M ShimizuY Ishikawa
Jul 1, 1987·Journal of Virological Methods·R Magar, J Lecomte
Dec 1, 1987·Nihon juigaku zasshi. The Japanese journal of veterinary science·M Shimizu, K Satou
Jun 1, 1967·Japanese Journal of Microbiology·T OmoriH Kurogi
Nov 1, 1993·Veterinary Microbiology·S R Bolin
Jan 1, 1991·Archives of Virology. Supplementum·J Brownlie
Jan 1, 1991·Archives of Virology. Supplementum·G CastrucciV Aldrovandi
Jan 1, 1991·Archives of Virology. Supplementum·S Belák, A Ballagi-Pordány
Jan 1, 1991·Archives of Virology. Supplementum·P M Roehe, M J Woodward

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Citations

Nov 26, 2002·Veterinary Parasitology·M YamasakiY Maede
May 1, 1997·The Veterinary Journal·J M Rodriguez
Aug 26, 1998·The Journal of Veterinary Medical Science·K UrunoT Nakane
Feb 5, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Motoshi Tajima, Edward J Dubovi
Sep 15, 2005·Oncogene·Nicolle M LinnerthRoger A Moorehead

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