Attempts to Detect Agrobacterium tumefaciens DNA in Crown-Gall Tumor Tissue.

Plant Physiology
D J Merlo, J D Kemp

Abstract

Primary and secondary crown gall tissue cultures were established from sunflower plants (Helianthus annuus, variety Mammoth Russian) wound-inoculated with Agrobacterium tumefaciens (Smith and Townsend) Conn strain B(6). Growth rates of tumor tissues and habituated healthy sunflower stem section tissues on basal medium lacking auxin and cytokinin were compared to those of healthy sunflower stem section tissue grown on the same medium with added phytohormones. No difference was detected in the thermal denaturation midpoints (74.8 C) and melting profiles in 25 mm sodium phosphate (pH 6.8), or the buoyant densities in cesium chloride equilibrium centrifugation (1.687 g cm(-3)), between deoxyribonucleic acids (DNAs) isolated from crude nuclear preparations of the four tissue types. No satellite DNA was observed in equilibrium centrifugation of unsheared plant DNAs.Heterologous DNA renaturation kinetic analyses were performed in 0.14 m sodium phosphate (pH 6.8) at 70 C. Thermal stability measurements of reassociated DNA revealed less than 1% of mismatched base pairs. Reannealing of sheared, denatured, radioactive A. tumefaciens B(6) DNA (molecular weight, 325,000 daltons) in the presence of a 5400-fold excess of sheared calf thymus, ...Continue Reading

References

Dec 15, 1975·Biochemical and Biophysical Research Communications·J D Kemp, D J Merlo
Mar 4, 1976·Biochimica Et Biophysica Acta·J D Kemp, D W Sutton
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M D ChiltonE W Nester
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·K A Drlicá, C I Kado
Jun 15, 1974·Journal of Molecular Biology·I ZaenenJ Schell
Aug 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·K P BaetckeS S Schwemmer
Aug 8, 1970·Life Sciences. Pt. 2: Biochemistry, General and Molecular Biology·B I Srivastava
Aug 24, 1970·Biochemical and Biophysical Research Communications·B I Srivastava, K C Chadha
Feb 14, 1968·Journal of Molecular Biology·J G Wetmur, N Davidson
Jan 6, 1969·Biochemical and Biophysical Research Communications·F QuétierE Guillé
Aug 1, 1959·The Journal of Biophysical and Biochemical Cytology·E RASCHR M KLEIN
Feb 1, 1965·Journal of Molecular Biology·F W STUDIER
Jul 15, 1957·Proceedings of the National Academy of Sciences of the United States of America·M MeselsonJ Vinograd
Sep 5, 1941·Science·P R White, A C Braun

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Citations

Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W B GurleyJ Callis
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·N Murai, J D Kemp
Jul 1, 1979·Plant Physiology·G K Lamppa, A J Bendich
May 3, 2013·American Journal of Infection Control·Charles E EdmistonBrian L Lewis
Dec 26, 1978·Biochemistry·M G MurrayW F Thompson

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