PMID: 6160122May 1, 1980Paper

Attempts to quantitate immunocytochemistry at the electron microscope level

The Histochemical Journal
J P KraehenbuhlG W Griffiths

Abstract

The ability to localize intracellular macromolecules in situ by high resolution techniques has been made possible by the development of antibody labelling of thin sections obtained either from tissues embedded in an hydrophilic matrix, or by ultracryotomy or from conventional plastic embedded tissue. When particle-tagged immunological reagents are used to visualize intracellular antigens, quantitative information can be obtained by combining particle counts with morphometric estimations of compartment volume. Various detection systems have been used successfully for quantitation, which include ferritin-conjugated antibodies, biotin-avidin-ferritin complexes and, more recently, gold-protein A conjugates. Examples of the use of these techniques the localization of secretory proteins in pancreatic exocrine cells, opsin and a large membrane protein in photoreceptor cells of frog retina, and contractile proteins in skeletal muscle are given. Quantitative data obtained by morphometric analysis, both in bovine and rat pancreatic exocrine cells, are compared with values assessed by biochemical methods.

References

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Citations

Oct 1, 1985·Developmental Biology·G M Wessel, D R McClay
Dec 1, 1989·Journal of Ultrastructure and Molecular Structure Research·S Nielsen, E I Christensen
Jun 27, 2013·Nature Neuroscience·Mahmood Amiry-Moghaddam, Ole Petter Ottersen
May 1, 1995·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·H C Mutasa
Jan 1, 1987·Ultrastructural Pathology·M M Silver, S A Hearn
Jan 1, 1995·Progress in Histochemistry and Cytochemistry·M Bendayan
Feb 24, 2001·Progress in Histochemistry and Cytochemistry·G Mayer, M Bendayan
Jul 1, 1989·Journal of Microscopy·A DahlströmP A Larsson
Jan 18, 2006·Journal of Biomedical Optics·Miriam S DrosteRoger Wepf
Dec 1, 1987·Gamete Research·G M WesselD R McClay

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