Attenuated cardiovascular hypertrophy and oxidant generation in response to angiotensin II infusion in glutaredoxin-1 knockout mice.

Free Radical Biology & Medicine
Markus M BachschmidReiko Matsui

Abstract

Glutaredoxin-1 (Glrx) is a thioltransferase that regulates protein S-glutathiolation. To elucidate the role of endogenous Glrx in cardiovascular disease, Glrx knockout (KO) mice were infused with angiotensin II (Ang II) for 6days. After Ang II infusion, body weight and blood pressure were similar between WT and Glrx KO mice. However, compared to WT mice, Glrx KO mice demonstrated (1) less cardiac and aortic medial hypertrophy, (2) less oxidant generation in aorta as assessed by dihydroethidium staining and nitrotyrosine, (3) decreased phosphorylation of Akt in the heart, and (4) less expression of inducible NOS in aorta and heart. In cultured embryonic fibroblasts from Glrx KO mice, S-glutathiolation of actin was enhanced and actin depolymerization was impaired after hydrogen peroxide stimulation compared with WT cells. Furthermore, oxidant generation in phorbol ester-stimulated fibroblasts and RAW 264.7 macrophage-like cells was lower with Glrx siRNA knockdown. These data indicate that Ang II-induced oxidant production and hypertrophic responses were attenuated in Glrx KO mice, which may result from impaired NADPH oxidase activation.

References

Jan 3, 1997·Biochemical and Biophysical Research Communications·T MorimatsuM Tamura
Feb 7, 1998·The Journal of Biological Chemistry·S BandyopadhyayR M Gronostajski
Apr 28, 1999·Free Radical Biology & Medicine·E B Meyer, W W Wells
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·H NunoiS Kanegasaki
Apr 12, 2001·The Journal of Biological Chemistry·M LundbergA Holmgren
Jun 9, 2001·The Journal of Biological Chemistry·V N GladyshevM F Lou
Apr 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jun WangP Boon Chock
Nov 5, 2003·The Journal of Clinical Investigation·Mitsutaka YamamotoJunichi Sadoshima
Jan 10, 2004·Antioxidants & Redox Signaling·Aristi Potamitou Fernandes, Arne Holmgren
May 5, 2004·The Journal of Biological Chemistry·Takeshi AdachiRichard A Cohen
Jun 9, 2004·Biochemical and Biophysical Research Communications·Mathias LundbergArne Holmgren
Mar 11, 2005·Canadian Journal of Physiology and Pharmacology·Rhian M TouyzErnesto L Schiffrin
Nov 22, 2005·Free Radical Biology & Medicine·Tuanzhu HaChuanfu Li
Jan 18, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nicolas ClavreulRichard A Cohen
Jun 30, 2006·Journal of Molecular and Cellular Cardiology·David R PimentelWilson S Colucci
Aug 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Niki L ReynaertYvonne M W Janssen-Heininger
Sep 19, 2006·American Journal of Respiratory Cell and Molecular Biology·Niki L ReynaertYvonne M W Janssen-Heininger
Nov 17, 2006·American Journal of Physiology. Cell Physiology·Victoria C TuQin M Chen
Nov 3, 2007·Journal of Molecular and Cellular Cardiology·Gautam MalikDipak K Das

❮ Previous
Next ❯

Citations

Oct 21, 2011·Antioxidants & Redox Signaling·David PimentelMarkus Michael Bachschmid
May 23, 2012·Antioxidants & Redox Signaling·Vikas KumarMarkus Michael Bachschmid
Jul 18, 2015·Expert Review of Proteomics·Jingwen JiangCanhua Huang
Jul 19, 2011·Trends in Molecular Medicine·Kai FriedrichsStephan Baldus
Feb 14, 2013·Obesity·Matthew J PickloMatthew I Jackson
Jul 26, 2011·Journal of Molecular and Cellular Cardiology·Bradford G Hill, Aruni Bhatnagar
Feb 19, 2013·Biochimica Et Biophysica Acta·Pietro Ghezzi
May 7, 2016·Circulation Journal : Official Journal of the Japanese Circulation Society·Yosuke WatanabeReiko Matsui
May 11, 2016·Proceedings of the National Academy of Sciences of the United States of America·Yosuke WatanabeReiko Matsui
Mar 2, 2016·Journal of Cardiovascular Pharmacology·Richard A CohenReiko Matsui
Oct 28, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yoshimitsu YuraReiko Matsui
Dec 10, 2019·Antioxidants & Redox Signaling·Reiko MatsuiMarkus Michael Bachschmid
Sep 20, 2020·International Journal of Molecular Sciences·Mannix BurnsMarkus M Bachschmid
Dec 14, 2016·Antioxidants & Redox Signaling·Di ShaoMarkus M Bachschmid
Jan 29, 2016·Molecular Medicine Reports·Lingxia PangLingjian Wu
Jan 1, 2021·Antioxidants·Lidia de BariTatiana Armeni
Mar 7, 2021·International Journal of Molecular Sciences·Rouven BerndtRené Rusch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomegaly

Cardiomegaly, known as an enlarged heart, is a multifactorial disease with different pathophysiological mechanisms. Hypertension, pregnancy, exercise-induced and idiopathic causes are some mechanisms of cardiomegaly. Discover the latest research of cardiomegaly here.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.