Attenuated Listeria monocytogenes as a Vaccine Vector for the Delivery of OMPW, the Outer Membrane Protein of Aeromonas hydrophila

Frontiers in Microbiology
Haijuan ZengQing Liu

Abstract

Listeria monocytogenes (LM) is a gram-positive facultative intracellular pathogen that could stimulate host to produce inflammatory response, cell-mediated immunity, and humoral immunity. In this study, an attenuated live vector vaccine for Aeromonas hydrophila (AH) named EGDeABdd-dat-ompW was successfully constructed using an attenuated vector named EGDeABdd, in which dal, dat, actA, and inlB genes were deleted from wild-type LM-EGDe. To construct EGDeABdd-dat-ompW, a recombinant plasmid pERL3-dat-ompW obtained by inserting the dat gene from EGDe and outer membrane protein gene ompW from AH into pERL3 plasmid was transformed into EGDeABdd cell. The safety and immunogenicity of EGDeABdd-dat-ompW as an attenuated vector vaccine for delivery of OMPW were assessed through analyzing invasion to Caco-2 cells and mice, cytokine production of macrophagocyte and mouse splenocytes, and T-cell proliferation of mouse splenocytes. Serum titers against AH and the immunoprotective effect of the vaccine to mice were also measured after intravenous injection with vaccine for four times. The results showed that the live vector vaccine EGDeABdd-dat-ompW for AH exhibited high attenuation in invading Caco-2 cells and mice than did EGDe. Real-time ...Continue Reading

References

Jan 13, 2010·Clinical Microbiology Reviews·J Michael Janda, Sharon L Abbott
Aug 25, 2012·Veterinary Immunology and Immunopathology·Biswajit MaitiIndrani Karunasagar
May 27, 2014·Frontiers in Cellular and Infection Microbiology·Laurence M Wood, Yvonne Paterson
Jan 15, 2015·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Dung T LeElizabeth M Jaffee
Mar 20, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nicola J MasonYvonne Paterson
Jul 15, 2017·Frontiers in Cellular and Infection Microbiology·Yan Yan JiaXin An Jiao
Oct 17, 2017·Frontiers in Cellular and Infection Microbiology·Yuelan YinXin'an Jiao
Feb 9, 2018·Frontiers in Microbiology·Joelle K SalazarMary Lou Tortorello
Aug 1, 2018·Frontiers in Cellular and Infection Microbiology·Showgy Y Ma'ayehStaffan G Svärd

❮ Previous
Next ❯

Citations

Dec 29, 2020·Frontiers in Microbiology·Fanzeng MengYuelan Yin
Oct 16, 2021·Frontiers in Cellular and Infection Microbiology·William Robert PownallAnna Oevermann

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Infection
flow cytometry
ELISA
Flow
serum collection

Software Mentioned

GraphPad Prism

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.

Related Papers

JAMA : the Journal of the American Medical Association
Tijdschrift Voor Diergeneeskunde
Iaira Boissevain
Microbes and Infection
Kevin W BruhnJeff F Miller
Revue française de transfusion et immuno-hématologie
G GerardJ Guimbretiere
© 2021 Meta ULC. All rights reserved