Attenuation of enoyl coenzyme A hydratase 1 expression in colorectal cancer cells using small interfering RNA inhibits cell proliferation and migration.

Molecular Medicine Reports
Qing-Mei ZhaoYu-Hao Zhang

Abstract

Colorectal cancer is one of the most commonly diagnosed types of cancer and is a leading cause of cancer-associated mortality worldwide. Short chain enoyl coenzyme A hydratase 1 (ECHS1) is an important gene involved in the mitochondrial fatty acid β-oxidation pathway. In addition, ECHS1 has been implicated in a variety of cancers, including breast, prostate, colon and liver cancer. The aim of the present study was to examine the expression of ECHS1 in the human HCT-8 colorectal cancer cell line. The results showed that ECHS1 expression was significantly increased in poorly-differentiated cells compared with that in well-differentiated cells. In order to further investigate the functions of ECHS1 in colorectal cancer cells, a stably transfected HCT-8 cell line expressing small interfering (si)RNA targeting the ECHS1 gene was established. The expression of the ECHS1 siRNA was found to reduce ECHS1 protein levels in ECHS1-silenced cells by >40%. Cell proliferation and cell migration of the siECHS1 cells were characterized using Cell Counting Kit-8 and Transwell assays, respectively, the results of which showed that the constitutive knockdown of the ECSH1 gene in HCT-8 cells significantly inhibited cell proliferation and migration....Continue Reading

References

Feb 18, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Xuequn ZhangFuchu He
Feb 19, 2013·FEBS Letters·Yan ChangWei-Na Zhang
Jul 3, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun ZhangJianwu Tang
Jul 25, 2013·Critical Reviews in Eukaryotic Gene Expression·Xiao-san ZhuQiu-hui Tan
Mar 4, 2014·Medical Gas Research·Lei ZhangZhongcong Xie
Mar 22, 2014·Molecular and Clinical Oncology·Guang-Jun ZhangShu-Sen Xia
Mar 22, 2014·Molecular and Clinical Oncology·Nuengruetai OrannapalaiSurasak Sangkhathat
Feb 1, 2015·Methods in Molecular Biology·Ilija CrncecRobert Eferl

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