Attenuation of human lung mast cell degranulation by bronchial epithelium

Allergy
W YangPeter Bradding

Abstract

Human lung mast cells (HLMC) lie in close proximity to the bronchial epithelium in asthma and adhere with high affinity to bronchial epithelial monolayers in vitro. We investigated the consequences of this adhesive interaction on HLMC activation in response to Fc epsilon RI cross-linking. Human lung mast cells were cultured with the bronchial epithelial cell line BEAS-2B or plastic control for either 30 min or 16 h and then activated with anti-IgE. Histamine was measured by radioenzymatic assay. After co-culture for 30 min, IgE-dependent histamine release from HLMC was identical on both BEAS-2B and plastic. After 16 h of co-culture, there was a marked decrease in constitutive and IgE-dependent histamine release from HLMC cultured on BEAS-2B compared with those cultured on plastic or fibronectin. In contrast, the Ca(2+)/ATPase inhibitor thapsigargin produced concentration-dependent histamine release that was significantly increased on BEAS-2B compared with plastic. IgE-dependent degranulation was not significantly affected by BEAS-2B-conditioned medium. BEAS-2B bronchial epithelial cells attenuate IgE-dependent but not thapsigargin-induced histamine release from HLMC. The differential effect with anti-IgE compared with thapsigar...Continue Reading

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Citations

May 10, 2005·American Journal of Respiratory and Critical Care Medicine·Christopher E BrightlingPeter Bradding
Sep 9, 2006·American Journal of Respiratory and Critical Care Medicine·Davinder KaurChristopher E Brightling
Jun 15, 2008·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Peter Bradding
Nov 17, 2015·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Peter Bradding, Greer Arthur
Jun 30, 2009·The Journal of Allergy and Clinical Immunology·Emily J SwindleDonna E Davies
Jan 25, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J M BrownE Mezey
May 12, 2015·European Journal of Pharmacology·Glenn Cruse, Peter Bradding
Sep 23, 2016·Expert Review of Vaccines·Susanna Esposito, Nicola Principi
May 22, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Benjamin J PatchellDelbert R Dorscheid
Apr 18, 2020·The European Respiratory Journal·Sara J BonviniMaria G Belvisi
Jun 14, 2019·Frontiers in Immunology·Philipp M HagemannZane Orinska
Aug 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fay HollinsPeter Bradding

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