Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy.

Nutrients
Ching-Ying HuangGuan-Jhong Huang

Abstract

The anti-inflammatory effect of hispolon has identified it as one of the most important compounds from Sanghuangporus sanghuang. The research objectives were to study this compound using an animal model by lipopolysaccharide (LPS)-induced acute lung injury. Hispolon treatment reduced the production of the pro-inflammatory mediator NO, TNF-α, IL-1β, and IL-6 induced by LPS challenge in the lung tissues, as well as decreasing their histological alterations and protein content. Total cell number was also reduced in the bronchoalveolar lavage fluid (BALF). Moreover, hispolon inhibited iNOS, COX-2 and IκB-α and phosphorylated IKK and MAPK, while increasing catalase, SOD, GPx, TLR4, AKT, HO-1, Nrf-2, Keap1 and PPARγ expression, after LPS challenge. It also regulated apoptosis, ER stress and the autophagy signal transduction pathway. The results of this study show that hispolon regulates LPS-induced ER stress (increasing CHOP, PERK, IRE1, ATF6 and GRP78 protein expression), apoptosis (decreasing caspase-3 and Bax and increasing Bcl-2 expression) and autophagy (reducing LC3 I/II and Beclin-1 expression). This in vivo experimental study suggests that hispolon suppresses the LPS-induced activation of inflammatory pathways, oxidative inju...Continue Reading

References

May 4, 2000·The New England Journal of Medicine·L B Ware, M A Matthay
Oct 1, 2003·Molecular and Cellular Biology·Sara B CullinanJ Alan Diehl
Dec 9, 2004·International Immunology·Kiyoshi Takeda, Shizuo Akira
Apr 6, 2006·The Journal of Clinical Investigation·Rajesh K ThimmulappaShyam Biswal
May 15, 2007·Oncogene·A S DhillonW Kolch
Aug 19, 2008·Biochimica Et Biophysica Acta·Eeva-Liisa Eskelinen, Paul Saftig
Feb 3, 2009·The Journal of Biological Chemistry·Truyen NguyenCecil B Pickett
Mar 28, 2009·American Journal of Respiratory and Critical Care Medicine·Murali ShyamsundarDaniel F McAuley
Apr 8, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Heng-Yuan ChangGuan-Jhong Huang
Jan 20, 2010·FEBS Letters·Chang Hwa JungDo-Hyung Kim
Feb 12, 2011·Cell Death and Differentiation·R KangD Tang
Jan 31, 2013·Biochimica Et Biophysica Acta·Benjamin BonneauGermain Gillet
Nov 16, 2013·The American Journal of Chinese Medicine·Yi-Chuan ChenGuan-Jhong Huang
Dec 30, 2014·Journal of Cellular Physiology·Genyuan Zhu, Amy S Lee
Aug 19, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Shaoying LiGuisheng Qian
May 4, 2016·Molecular Cancer Research : MCR·Sarah FogartyD Grahame Hardie
May 27, 2016·The Journal of Biological Chemistry·Ananthalakshmy SundararamanAnnapoorni Rangarajan
Nov 20, 2016·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Kim M Keppler-NoreuilJulian A Martinez-Agosto
Jan 20, 2017·Oxidative Medicine and Cellular Longevity·Ravirajsinh N JadejaSandeep Khurana
Feb 9, 2017·International Journal of Molecular Sciences·Wang-Ching LinGuan-Jhong Huang
Nov 11, 2017·The American Journal of Chinese Medicine·Ming-Shun WuYen-Chou Chen
Mar 30, 2018·The American Journal of Chinese Medicine·Heng-Yuan ChangGuan-Jhong Huang
Nov 15, 2018·Oxidative Medicine and Cellular Longevity·Jeongmin ParkHun Taeg Chung

❮ Previous
Next ❯

Citations

Jun 7, 2021·Journal of Applied Toxicology : JAT·Yan-Ping LiuZhao-Hui Zhang
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kuan-Fu LiaoSheng-Chun Chiu

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
bronchoalveolar
lavage
ELISA
enzyme-linked immunasorbent
Fluorescence
xenograft
nuclear translocation

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms