Attenuation of MET-mediated migration and invasion in hepatocellular carcinoma cells by SOCS1

World Journal of Gastroenterology : WJG
Yirui GuiSubburaj Ilangumaran

Abstract

To investigate the role of suppressor of cytokine signaling 1 (SOCS1) in regulating MET-mediated invasive potential of hepatocellular carcinoma (HCC) cells. Stable derivatives of mouse (Hepa1-6) and human (hep3B, HepG2) HCC cell lines expressing SOCS1 or control vector were evaluated for their ability to migrate towards hepatocyte growth factor (HGF) in the transwell migration assay, invade extracellular matrix in response to HGF stimulation in a 3-D invasion assay by confocal microscopy, and to undergo anchorage-independent proliferation in semisolid agar. Following intravenous and intrasplenic inoculation into NOD.scid.gamma mice, the ability of Hepa cells to form othotopic tumors was evaluated. Following HGF stimulation of Hepa and Hep3B cells, expression of proteins implicated in epithelial-to-mesenchymal transition was evaluated by western blot and qRT-PCR. SOCS1 expression in mouse and human HCC cells inhibited HGF-induced migration through matrigel. In the 3-D invasion assay, HGF stimulation induced invasion of HCC cells across type-I collagen matrix, and SOCS1 expression significantly reduced the depth of invasion. SOCS1 expression also reduced the number and size of colonies formed by anchorage-independent growth in se...Continue Reading

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Citations

Dec 19, 2018·International Journal of Molecular Sciences·Sunny Trivedi, Michelle Starz-Gaiano
Jan 4, 2019·Journal of Translational Medicine·Bodo C Melnik, Gerd Schmitz
Mar 3, 2020·Frontiers in Cell and Developmental Biology·Haiyu WangZujiang Yu
Apr 27, 2021·Frontiers in Oncology·Kamila KitowskaRafal Sadej

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Methods Mentioned

BETA
ubiquitination
Protein Assay
confocal microscopy
biopsies

Software Mentioned

Nanozoomer Digital Pathology ( NDP )
NIH ImageJ
NDP

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