Attenuation of Na/K-ATPase/Src/ROS amplification signal pathway with pNaktide ameliorates myocardial ischemia-reperfusion injury

International Journal of Biological Macromolecules
Hua LiLingmei Qian

Abstract

Oxidative stress plays an important role in myocardial ischemia-reperfusion (I/R) injury. And pNaKtide is known to inhibit Na/K-ATPase/Src/reactive oxygen species (ROS) amplification signaling. Accordingly, we aimed to investigate the effect of pNaKtide on myocardial I/R injury. We first determine the effect of pNaKtide on hypoxia- or cobalt chloride-induced injury in embryonic heart-derived H9c2 cells via measuring lactate dehydrogenase (LDH) level and trypan blue stain assay. In addition, TUNEL stain assay and western blot analysis of cleaved-PARP and cleaved-caspase3 were performed to detect apoptosis level. Meanwhile, ROS accumulation was assessed by dichlorofluorescin diacetate (DCFH-DA) assay. Then we conducted cell counting kit-8 (CCK-8) and flow cytometry to examine cell proliferation and cell cycle respectively. We next generated rat I/R model and determined the effect of pNaKtide by measuring serum LDH and evaluating heart pathology. At last, the activities of Src and ERK1/2 were examined via western blot to clarify molecular mechanism. In vitro, pNaKtide exposure significantly attenuated the H9c2 cells death and ROS accumulation induced by hypoxia or cobalt chloride. And no significant effect was detected on cell cyc...Continue Reading

Citations

Oct 5, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Shaohui Zhang, Yongliang Zhao
Jun 27, 2020·Journal of the American Society of Nephrology : JASN·Komal SodhiJoseph I Shapiro
Oct 29, 2020·Oxidative Medicine and Cellular Longevity·Li ZhangLingmei Qian
Mar 23, 2021·Acta Physiologica·Laura C KutzSandrine V Pierre
May 6, 2021·Stroke; a Journal of Cerebral Circulation·Halvor Osterby GuldbrandsenVladimir V Matchkov
May 27, 2021·American Journal of Hypertension·Xiaohong JiHongjuan Ding
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ying FuYuou Teng

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