PMID: 7537143Jan 1, 1995Paper

Attenuation of parasite cAMP levels in T. cruzi-host cell membrane interactions in vitro

The Journal of Eukaryotic Microbiology
B F von KreuterC A Santos-Buch

Abstract

Previous investigations have shown that the adhesion of T. cruzi plasma membrane vesicles (PMV) to monolayers of host cell myoblasts and to immobilized heart muscle sarcolemma membranes (PAM) on polyacrylamide beads is mediated by the interaction of T. cruzi attachment sites with the muscarinic cholinergic and beta-adrenergic receptors of the host cell membrane. It has also been shown that this interaction is blunted by the specific antagonists of the mammalian receptors atropine and propranol, respectively. In the studies reported here, PAM also rapidly attached to swimming T. cruzi trypomastigotes in a complex, concentration-dependent fashion and binding isotherms showed that the equilibrium between free and bound PAM is rapidly reached within 2 minutes of incubation in physiologically balanced salt solutions. In this time frame, trypomastigote cAMP levels are significantly reduced from steady state values within 30 seconds of the addition of PAM in a buffer system containing a diesterase inhibitor. Maximal attenuation of cAMP levels was measured between 1 and 2 minutes of the addition of PAM to T. cruzi trypomastigotes. The degree of cAMP level attenuation was reduced by blocking PAM attachment with either atropine or propra...Continue Reading

References

Sep 1, 1988·Molecular and Biochemical Parasitology·B F von KreuterC A Santos-Buch
Jun 1, 1989·Proceedings of the Society for Experimental Biology and Medicine·B F Von KreuterC A Santos-Buch
Sep 1, 1989·Molecular and Biochemical Parasitology·M L GómezM T Téllez-Iñón
Aug 1, 1986·The Biochemical Journal·C D EisenschlosM M Flawiá
Dec 31, 1987·Biochemical and Biophysical Research Communications·L KhachatrianA Howlett
Sep 1, 1986·The American Journal of Tropical Medicine and Hygiene·M Sadigursky, C I Brodskyn
Aug 1, 1993·Cell Biology International·T Eschenhagen
Apr 1, 1959·Biulleten' eksperimental'noĭ biologii i meditsiny·E E PULATOVA

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Citations

Jul 8, 2011·Clinical Microbiology Reviews·Antonio R L TeixeiraNadjar Nitz

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