Attenuation of Postoperative Acute Liver Failure by Mesenchymal Stem Cell Treatment Due to Metabolic Implications

Annals of Surgery
Hans-Michael TautenhahnBruno Christ

Abstract

To prevent posthepatectomy acute liver failure after extended resection by treatment with mesenchymal stem cells (MSCs). Liver tumors often require extended liver resection, overburdening metabolic and regenerative capacities of the remnant organ. Resulting dysfunction and failure may be improved by the proregenerative characteristics of MSCs. Extended liver resection was performed in (DPPIV)-deficient F344-Fischer rats. Wild-type animals served as donors of peritoneal adipose-derived MSCs. These were predifferentiated in vitro into hepatocytic cells and delivered to the liver by splenic application. Liver-related blood parameters (international normalized ratio, bilirubin, aspartate aminotransferase, alanine aminotransferase) and liver histology (hematoxylin-eosin, Sudan III) were determined to monitor liver function. Metabolic changes were assessed by metabolomic analyses in the remnant liver and the serum. Liver damage and regeneration were quantified by determination of the apoptotic and proliferation rates. MSCs supported survival after partial hepatectomy. They decreased liver-related blood parameters indicative for the improvement of liver function. The extensive lipid accumulation in hepatocytes illustrating the metabol...Continue Reading

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Citations

Dec 19, 2017·Frontiers in Physiology·Bruno ChristNavina Waschinsky
Jul 10, 2019·Stem Cell Research & Therapy·Chenxia HuLanjuan Li
Jun 15, 2019·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Chenxia Hu, Lanjuan Li
Feb 16, 2020·Cellular and Molecular Life Sciences : CMLS·Saisai WeiXiujun Cai
Jul 11, 2019·Current Transplantation Reports·Aylin AcunBasak E Uygun
Nov 7, 2019·Journal of Cellular and Molecular Medicine·Chenxia HuLanjuan Li
Aug 26, 2018·Stem Cell Research & Therapy·Yong-Hong WangHong Tang
Aug 28, 2021·Journal of Reproductive Immunology·Narges NouriMehdi Yousefi

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Methods Mentioned

BETA
density gradient centrifugation
flow cytometry
Flow Injection
metabolomic profiling

Software Mentioned

MetIQ
ImageJ

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