Attenuation of rat lung isograft reperfusion injury with a combination of anti-ICAM-1 and anti-beta2 integrin monoclonal antibodies

Transplantation
S R DeMeesterG A Patterson

Abstract

Four different combinations of monoclonal antibodies against rat ICAM-1, CD-11a, and CD-18 were utilized to determine the relative importance of LFA-1, Mac-1, and ICAM-1 in a rat model of severe lung allograft reperfusion injury. Negative control animals were given phosphate buffered saline (the carrier solution for the antibodies), while positive control animals were rendered neutropenic by the administration of a polyclonal mouse IgG. Antibodies were given with the donor lung flush, prior to left lung graft reperfusion, or both. Isolated graft function was determined 24 hr after implantation by arterial blood gas (ABG), and after sacrifice the native and transplanted lungs underwent bronchoalveolar lavage for alveolar protein quantitation, cell count and differential, and myeloperoxidase assay. Additionally, whole lung homogenates were assayed for myeloperoxidase activity. We found that the combination of anti-ICAM-1 (1 mg/kg) added to the donor lung flush, and anti-CD11a, anti-CD18, and anti-ICAM-1 (2 mg/kg i.v. of each) given to the recipient prior to reperfusion, resulted in significantly improved lung graft pAO2 by ABG, and decreased alveolar protein, cell count, and myeloperoxidase activity compared with control animals....Continue Reading

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Citations

Sep 2, 1998·The Annals of Thoracic Surgery·I NagahiroG A Patterson
Aug 24, 1999·Transplantation Proceedings·M WatanabeK Yasuda
Aug 19, 1999·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·N TomizawaY Morishita
Oct 9, 1999·Clinical Transplantation·B BewigJ Cremer
Feb 18, 2003·American Journal of Respiratory and Critical Care Medicine·Marc de PerrotShaf Keshavjee
Jul 16, 2005·American Journal of Respiratory and Critical Care Medicine·David S WilkesHerbert Y Reynolds
Feb 28, 2001·Journal of Cardiac Surgery·C B Huddleston, E N Mendeloff
May 25, 2007·Archives of Physiology and Biochemistry·Alexander V OvechkinAndrew M Roberts
Jul 13, 2011·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Tillmann CyrusGregory M Lanza
Jan 18, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Thomas D DziublaVladimir R Muzykantov
Oct 8, 2005·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Mark L BarrUNKNOWN ISHLT Working Group on Primary Lung Graft Dysfunction
Sep 22, 1999·Gene Therapy·N GiannoukakisP Robbins
Jul 25, 2003·American Journal of Physiology. Cell Physiology·Silvia MuroMichael Koval
Nov 12, 2019·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Xingyu WangLucas Liaudet
Nov 9, 2000·American Journal of Respiratory and Critical Care Medicine·P EnkhbaatarY Yamaguchi
Oct 1, 1998·The American Journal of Physiology·E N AtochinaV R Muzykantov

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