Attenuation of TRPV1 by AMG-517 after nerve injury promotes peripheral axonal regeneration in rats

Molecular Pain
Juan BaiXia-Qing Li

Abstract

Aims The main objective was to investigate the effects of the transient receptor potential cation channel subfamily V member 1 (TRPV1) on nerve regeneration following sciatic transection injury by functional blockage of TRPV1 using AMG-517, a specific blocker of TRPV1. Methods AMG-517 was injected into the area surrounding ipsilateral lumbar dorsal root ganglia 30 min after unilateral sciatic nerve transection. The number of sciatic axons and the expression of growth-associated protein-43 (GAP-43) and glial fibrillary acidic protein was examined using semithin sections, Western blot, and immunofluorescence analyses. Results Blockage of TRPV1 with AMG-517 markedly promoted axonal regeneration, especially at two weeks after sciatic injury; the number of axons was similar to the uninjured control group. After sciatic nerve transection, expression of glial fibrillary acidic protein was decreased and GAP-43 was increased at the proximal stump. However, the expression of both glial fibrillary acidic protein and GAP-43 increased significantly in AMG-517-treated groups. Conclusions TRPV1 may be an important therapeutic target to promote peripheral nerve regeneration after injury.

References

Oct 25, 1996·The Journal of Biological Chemistry·C GambyL Baizer
Feb 1, 1997·Trends in Neurosciences·L I Benowitz, A Routtenberg
Jun 14, 2003·The EMBO Journal·Marco CapriniAntonio Ferrer-Montiel
Jun 21, 2007·Annual Review of Biochemistry·Kartik Venkatachalam, Craig Montell
Oct 17, 2007·Bioorganic & Medicinal Chemistry Letters·Xianghong WangMark H Norman
Oct 22, 2008·Molecular and Cellular Neurosciences·Alessandra MusellaDiego Centonze
Nov 18, 2008·Canadian Journal of Physiology and Pharmacology·Shi-Yin GuoZhen-Qiu Sun
Aug 5, 2009·Neurochemistry International·Johanna El Andaloussi-LiljaAnna Forsby
Oct 16, 2010·European Journal of Pharmacology·Xiu-Ju LuoYuan-Jian Li
Oct 5, 2011·Current Opinion in Neurology·Larry I Benowitz, Phillip G Popovich
Apr 2, 2013·Plastic and Reconstructive Surgery·Ying YanPhilip J Johnson
Sep 18, 2015·Science Translational Medicine·Jacob D BrownWilliam P Dillon
Feb 18, 2016·Neural Plasticity·R Ramírez-BarrantesP Olivero
Feb 10, 2017·Scientific Reports·Shih-Tien HsuYueh-Sheng Chen

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
light microscopy
electrophoresis
X-ray

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