Attractive design: an elution solvent optimization platform for magnetic-bead-based fractionation using digital microfluidics and design of experiments

Analytical Chemistry
Nelson M LafrenièreAaron R Wheeler

Abstract

There is great interest in the development of integrated tools allowing for miniaturized sample processing, including solid phase extraction (SPE). We introduce a new format for microfluidic SPE relying on C18-functionalized magnetic beads that can be manipulated in droplets in a digital microfluidic platform. This format provides the opportunity to tune the amount (and potentially the type) of stationary phase on-the-fly, and allows the removal of beads after the extraction (to enable other operations in same device-space), maintaining device reconfigurability. Using the new method, we employed a design of experiments (DOE) operation to enable automated on-chip optimization of elution solvent composition for reversed phase SPE of a model system. Further, conditions were selected to enable on-chip fractionation of multiple analytes. Finally, the method was demonstrated to be useful for online cleanup of extracts from dried blood spot (DBS) samples. We anticipate this combination of features will prove useful for separating a wide range of analytes, from small molecules to peptides, from complex matrices.

References

Jun 15, 1997·Analytical Chemistry·A F HühmerC Schôneich
Jul 13, 2000·Journal of Chromatographic Science·J S JaniszewskiH G Fouda
Oct 26, 2007·Journal of Mass Spectrometry : JMS·Simon EkströmThomas Laurell
Mar 29, 2008·Lab on a Chip·Irena Barbulovic-NadAaron R Wheeler
May 17, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Vivienne N LukAaron R Wheeler
Oct 25, 2008·Science·Aaron R Wheeler
Nov 22, 2008·Lab on a Chip·Ramakrishna SistaVamsee Pamula
Nov 22, 2008·Lab on a Chip·Ramakrishna S SistaVamsee K Pamula
Sep 24, 2009·Journal of Pharmaceutical Sciences·Michael H AbrahamWilliam E Acree
May 14, 2010·Analytical Chemistry·Arun AroraAndreas Manz
Dec 28, 2010·Analytical Biochemistry·Bor-Han ChuehRichard N Zare
Jun 10, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Sam H AuAaron R Wheeler
Aug 27, 2011·Lab on a Chip·Mais J JebrailAaron R Wheeler
May 17, 2012·Proteomics·Vivienne N LukAaron R Wheeler
Jun 5, 2012·Biomicrofluidics·Lindsey K FiddesAaron R Wheeler
Sep 12, 2012·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Sirisha EmaniSitaram M Emani
Sep 22, 2012·Journal of Chromatography. a·Pamela N NgeAdam T Woolley
Sep 28, 2012·Analytical Chemistry·Alphonsus H C NgAaron R Wheeler
Dec 5, 2012·Biosensors & Bioelectronics·Steve C C ShihAaron R Wheeler
Feb 26, 2014·Nature Communications·Irwin A EydelnantAaron R Wheeler
Jul 25, 2014·Analytical Chemistry·Ningsi MeiRichard Oleschuk
Jul 31, 2014·Analytical and Bioanalytical Chemistry·Maria-Nefeli TsaloglouHywel Morgan
Nov 14, 2014·Critical Reviews in Analytical Chemistry·Wan Aini Wan IbrahimHassan Y Aboul-Enein

❮ Previous
Next ❯

Citations

Dec 10, 2015·Analytical Chemistry·German A Gomez-RiosJanusz Pawliszyn
Nov 26, 2015·Analytical Chemistry·Damith E W PatabadigeChristopher T Culbertson
Oct 5, 2016·Analytical Chemistry·Brendon SealeAaron R Wheeler
Jan 3, 2020·Lab on a Chip·Junjie ZhongDavid Sinton
Jul 2, 2021·Electrophoresis·Hao ChengMing Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.