Atypical transcription of microRNA gene fragments.

Nucleic Acids Research
Jin Song GaoBharat Ramratnam

Abstract

MicroRNAs (miRNAs) are short ( approximately 22 nt) RNAs that impact gene expression by sequence-specific interactions with messenger RNA or promoter sequences of genomic DNA. Ectopic expression of miRNAs can be accomplished by placing fragments of the corresponding miRNA precursor under the control of RNA polymerase II or III (RNAP II/III). Here, we report that, in the absence of exogenous promoters, DNA fragments incorporating miRNA precursors can be delivered directly into a variety of human cells and give rise to the corresponding mature miRNA. Notably, the transcription of these miRNA DNA fragments appears resistant to conventional inhibitors of RNAP I/II/III activity. Taken together, our findings suggest the existence of a previously unrecognized atypical transcription program for miRNA precursor sequences.

References

Sep 26, 2003·Nature·Yoontae LeeV Narry Kim
Dec 6, 2003·Science·Chang-Zheng ChenDavid P Bartel
Feb 18, 2004·Nucleic Acids Research·Daniel BodenBharat Ramratnam
Sep 17, 2004·The EMBO Journal·Yoontae LeeV Narry Kim
Oct 27, 2004·The Journal of Biological Chemistry·Christine EsauRichard Griffey
Jun 21, 2005·Nature Genetics·Isaac BentwichZvi Bentwich
Nov 14, 2006·Nature Structural & Molecular Biology·Glen M BorchertBeverly L Davidson
Oct 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Harpreet Kaur SainiAnton James Enright
Jan 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Robert F PlaceRajvir Dahiya
May 20, 2008·Nucleic Acids Research·Niall S KennethRobert J White
Dec 6, 2008·Genes & Development·Fatih OzsolakDavid E Fisher
Apr 21, 2009·Nature Genetics·Ryan J TaftJohn S Mattick
Jul 9, 2009·Bioinformatics·David LangenbergerPeter F Stadler
Oct 22, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Shelly BeerDirk Grimm

❮ Previous
Next ❯

Citations

Aug 23, 2011·Journal of Biosciences·Ashwani JhaRavi Shankar
Feb 18, 2011·The Journal of Biological Chemistry·Rong HuXiang-Hang Luo
Dec 6, 2011·Biology Direct·Jittima PiriyapongsaNeil R Smalheiser
Oct 28, 2015·Cardiovascular Research·Sergii KyrychenkoNatalia Shirokova
Oct 28, 2010·Genomics·Brian C Schanen, Xiaoman Li
Aug 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kazuhiro IshibashiEtsuko Katoh
May 14, 2014·Proceedings of the National Academy of Sciences of the United States of America·Alexander J F EganJean-Pierre Simorre
Jan 19, 2019·Non-coding RNA·Irena Misiewicz-KrzeminskaNorma C Gutiérrez

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
transfection
GeneScreen
transfections
immunoprecipitation

Software Mentioned

SAS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.