PMID: 9422605Jan 9, 1998Paper

AtzC is a new member of the amidohydrolase protein superfamily and is homologous to other atrazine-metabolizing enzymes

Journal of Bacteriology
M J SadowskyL P Wackett

Abstract

Pseudomonas sp. strain ADP metabolizes atrazine to cyanuric acid via three plasmid-encoded enzymes, AtzA, AtzB, and AtzC. The first enzyme, AtzA, catalyzes the hydrolytic dechlorination of atrazine, yielding hydroxyatrazine. The second enzyme, AtzB, catalyzes hydroxyatrazine deamidation, yielding N-isopropylammelide. In this study, the third gene in the atrazine catabolic pathway, atzC, was cloned from a Pseudomonas sp. strain ADP cosmid library as a 25-kb EcoRI DNA fragment in Escherichia coli. The atzC gene was further delimited by functional analysis following transposon Tn5 mutagenesis and subcloned as a 2.0-kb EcoRI-AvaI fragment. An E. coli strain containing this DNA fragment expressed N-isopropylammelide isopropylamino hydrolase activity, metabolizing N-isopropylammelide stoichiometrically to cyanuric acid and N-isopropylamine. The 2.0-kb DNA fragment was sequenced and found to contain a single open reading frame of 1,209 nucleotides, encoding a protein of 403 amino acids. AtzC showed modest sequence identity of 29 and 25%, respectively, to cytosine deaminase and dihydroorotase, both members of an amidohydrolase protein superfamily. The sequence of AtzC was compared to that of E. coli cytosine deaminase in the regions co...Continue Reading

References

Oct 1, 1985·The Biochemical Journal·A M CookR Hütter
Dec 15, 1982·The Biochemical Journal·K JutziR Hütter
Dec 1, 1994·Biodegradation·R C WyndhamM C Peel
Mar 1, 1997·Applied and Environmental Microbiology·K L Boundy-MillsM J Sadowsky
Apr 1, 1995·Applied and Environmental Microbiology·R T MandelbaumL P Wackett
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T YamadaT Kosuge

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Citations

Mar 7, 2003·Canadian Journal of Microbiology·David M StamperOlli H Tuovinen
Jul 12, 2005·Pest Management Science·Raquel Santiago-MoraAntonio R Franco
Jul 27, 2006·FEMS Microbiology Ecology·Anne KersantéFrançoise Binet
Jan 12, 2007·Journal of Environmental Quality·Dale L Shaner, W Brien Henry
Oct 27, 2006·FEMS Microbiology Ecology·Daniel Smith, David E Crowley
Feb 2, 2008·Environmental Microbiology·Marion DeversFabrice Martin-Laurent
Mar 29, 2014·Applied Microbiology and Biotechnology·Medhat RehanMartin Hofrichter
Sep 30, 2000·Applied and Environmental Microbiology·J L SeffernickL P Wackett
Aug 30, 2002·Applied and Environmental Microbiology·Jennifer L SeffernickLawrence P Wackett
Dec 7, 2005·FEMS Microbiology Ecology·Daniel SmithDavid E Crowley
Sep 24, 2004·Proteins·Ori Shachar, Michal Linial
Apr 24, 2007·Angewandte Chemie·Miguel D ToscanoDonald Hilvert
Nov 1, 2002·FEMS Microbiology Ecology·M Pilar Garcillán-Barcia, Fernando de la Cruz
Mar 6, 2010·Journal of Applied Microbiology·N Udiković-KolićF Martin-Laurent
Feb 20, 2010·Applied Microbiology and Biotechnology·Koji Satsuma
Feb 6, 2016·Environmental Monitoring and Assessment·Anup Kumar, Neera Singh
May 16, 2013·Journal of Environmental Quality·Heather L TylerMatthew T Moore
Jun 6, 2003·Applied and Environmental Microbiology·Isaac FrucheyLawrence P Wackett
Feb 3, 1999·Annals of the New York Academy of Sciences·L P Wackett
Aug 2, 2008·FEMS Microbiology Letters·Kenichi YamazakiTai Uchimura
Jun 4, 2005·Pest Management Science·Juan C López-GutiérrezFabrice Martin-Laurent
Mar 8, 2000·Bioscience, Biotechnology, and Biochemistry·M WakayamaM Moriguchi
Apr 27, 2002·Applied and Environmental Microbiology·Susana I de Azevedo WäschThomas Leisinger
Feb 4, 2010·Pest Management Science·L Jason KrutzSteven J Thomson
Jun 1, 2004·FEMS Microbiology Ecology·Fabrice Martin-LaurentGuy Soulas
Jun 30, 2011·Journal of Environmental Quality·Chung-Ho LinHarold E Garrett
Oct 19, 2012·Applied Microbiology and Biotechnology·Nikolina Udiković-KolićFabrice Martin-Laurent
Sep 17, 2013·Journal of Bacteriology·Anthony G DodgeLawrence P Wackett
Apr 7, 2011·Applied Microbiology and Biotechnology·Nikolina Udiković-KolićFabrice Martin-Laurent
Mar 12, 2015·Acta Crystallographica. Section D, Biological Crystallography·T S PeatC Scott

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