Au nanoparticle decorated graphene nanosheets for electrochemical immunosensing of p53 antibodies for cancer prognosis

The Analyst
Reda ElshafeyAna C Tavares

Abstract

The accurate quantification of the level of p53 antibodies in serum is crucial for cancer prognosis. We report a novel and sensitive label-free immunosensor based on gold nanoparticles (Au NPs) self-assembled onto electrochemically reduced graphene oxide (ERGO) for the detection of p53 antibodies. An electrografted p-aminophenol organic layer was used to immobilize graphene oxide (GO) onto the surface of screen printed carbon electrodes (SPCE). The Au NP/ERGO hybrid interface provides a large surface area for the effective immobilization of p53 antigens, as well as it ascertains the bioactivity and stability of immobilized p53 antigens. Scanning electron microscope, Raman and X-ray photoelectron spectroscopies were used to monitor the sensor fabrication and cyclic voltammetry was used to quantify the extent of Au NPs' surface coverage by p53 antigens. Square wave voltammetry (SWV) of a [Fe(CN)6](3-/4-) couple was employed to investigate the immunosensor fabrication and to monitor the binding events between p53 antigens and p53 antibodies. Under optimized experimental conditions, the biosensor displayed good sensitivity and specificity. The p53 antibodies were detected in a concentration as low as 0.088 pg mL(-1) with a linear r...Continue Reading

References

Jul 2, 1992·Nature·D P Lane
May 1, 1996·Genes & Development·L J Ko, C Prives
Nov 1, 2000·Breast Cancer Research : BCR·S Metcalfe Jo Milner A
Dec 1, 2000·Nature·B VogelsteinA J Levine
Sep 12, 2001·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·M CioffiA M Molinari
Dec 4, 2001·Journal of Immunological Methods·Jean Michel PortefaixMaguy Del Rio
Mar 14, 2003·Nature·Sam Hanash
Feb 3, 2005·Chemical Communications : Chem Comm·Isabelle ArnaudHubert H Girault
Feb 7, 2006·Biosensors & Bioelectronics·Avraham Rasooly, James Jacobson
Jan 16, 2007·Analytical Chemistry·Rafael SzamockiAlexander Kuhn
Jun 21, 2007·Analytical Chemistry·Adriano AmbrosiArben Merkoçi
Dec 25, 2007·Nano Letters·Konstantin N KudinRoberto Car
Mar 5, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Karen S AndersonDaniel Cramer
Jul 22, 2011·Chemical Reviews·Sunil K Arya, Shekhar Bhansali
Jan 13, 2012·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Jul 28, 2012·ACS Nano·Bhaskara V ChikkaveeraiahXiaoyuan Chen
Jun 21, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Abdelmoneim MarsArben Merkoçi

❮ Previous
Next ❯

Citations

Sep 28, 2016·ACS Applied Materials & Interfaces·Zhaoli GaoA T Charlie Johnson
Feb 23, 2017·Analytica Chimica Acta·Christos Kokkinos, Anastasios Economou
Sep 24, 2018·Journal of Nanobiotechnology·Janire Peña-BahamondeDebora F Rodrigues
Jun 21, 2017·Scientific Reports·Peter D BurbeloIlias Alevizos
Sep 14, 2019·Talanta·Akbar KhanmohammadiHasan Bagheri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosensors for Cancer Detection

Biosensors are devices that are designed to detect a specific biological analyte by essentially converting a biological entity (ie, protein, DNA, RNA) into an electrical signal that can be detected and analyzed. The use of biosensors in cancer detection and monitoring holds vast potential. Biosensors can be designed to detect emerging cancer biomarkers and to determine drug effectiveness at various target sites. Biosensor technology has the potential to provide fast and accurate detection, reliable imaging of cancer cells, and monitoring of angiogenesis and cancer metastasis, and the ability to determine the effectiveness of anticancer chemotherapy agents.