AUF1-like protein binds specifically to DAS cis-acting element that regulates mouse alpha-fetoprotein gene expression

Journal of Cellular Biochemistry
Ruiqing JiaoJen-fu Chiu

Abstract

Alpha-fetoprotein (AFP) is one of the major serum proteins in the early life of mammals. We have previously identified a novel cis-acting element designated as DAS at the 5'-flanking region of the AFP gene and demonstrated that the DAS sequence can be specifically recognized by nuclear protein DAP-II in AFP-producing hepatoma cells and retinoic acid (RA)-induced AFP-producing F9 cells. In this study, we used DNA affinity chromatography to purify the DAP-II proteins from the nuclear extracts (NE) of RA-treated F9 cells. The purified DAP-II complex mainly contained five proteins, with molecular weights of 45, 42, 32, 30, and 20 kDa, respectively. The identification of these proteins was determined by MALDI-TOF mass spectrometric analysis and a database search. These proteins were found to belong to the AUF1 RNA-binding protein family. Protein (30 kDa), one of five proteins in an isolated DAP-II complex, was matched with amino acid sequence highly similar to muAUF1-3. The expression of this protein is inducible by RA, and the pattern of the protein expression is the same as DAP-II proteins in F9 cells after treatment with RA during differentiation. Our results suggest that the 30-kDa protein is a novel isoform of AUF1 family and i...Continue Reading

References

Sep 11, 1979·Nucleic Acids Research·J F ChiuP Commer
Jul 16, 1990·Biochemical and Biophysical Research Communications·J M DongJ F Chiu
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L Muglia, L B Rothman-Denes
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S G Widen, J Papaconstantinou
Mar 1, 1988·Molecular and Cellular Biology·R GodboutS M Tilghman
Oct 1, 1989·Molecular and Cellular Biology·M H FeuermanS M Tilghman
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J T Kadonaga, R Tjian
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·K ArtztF Jacob
Apr 30, 1984·Biochemical and Biophysical Research Communications·G J Cote, J F Chiu
Jul 1, 1994·Molecular and Cellular Endocrinology·Y LiuJ F Chiu
Nov 30, 1994·Biochemical and Biophysical Research Communications·Y LiuJ F Chiu
Dec 1, 1995·Journal of Cellular Physiology·T NakamakiH P Koeffler
Jan 1, 1993·Annual Review of Biochemistry·G DreyfussC G Burd
Jan 1, 1993·Methods in Enzymology·D B Tully, J A Cidlowski
Nov 1, 1995·Trends in Biochemical Sciences·C Y Chen, A B Shyu
May 24, 1996·The Journal of Biological Chemistry·C T DeMaria, G Brewer
Jul 1, 1996·Molecular and Cellular Biology·L GalarneauL Bélanger
Jan 1, 1997·Critical Reviews in Eukaryotic Gene Expression·H ChenJ F Chiu
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·L A HanakahiN Maizels
Feb 28, 1998·The Journal of Biological Chemistry·G A ApergisJ Locker
Feb 5, 1999·Progress in Nucleic Acid Research and Molecular Biology·G M Wilson, G Brewer
Jul 23, 2003·Journal of Cellular Biochemistry·Qing-Yu He, Jen-Fu Chiu

❮ Previous
Next ❯

Citations

May 31, 2011·Frontiers in Bioscience (Landmark Edition)·Beth E Zucconi, Gerald M Wilson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.