Augmentation of Ca(2+) signaling in astrocytic endfeet in the latent phase of temporal lobe epilepsy

Frontiers in Cellular Neuroscience
Karolina SzokolErlend A Nagelhus

Abstract

Astrocytic endfeet are specialized cell compartments whose important homeostatic roles depend on their enrichment of water and ion channels anchored by the dystrophin associated protein complex (DAPC). This protein complex is known to disassemble in patients with mesial temporal lobe epilepsy and in the latent phase of experimental epilepsies. The mechanistic underpinning of this disassembly is an obvious target of future therapies, but remains unresolved. Here we show in a kainate model of temporal lobe epilepsy that astrocytic endfeet display an enhanced stimulation-evoked Ca(2+) signal that outlast the Ca(2+) signal in the cell bodies. While the amplitude of this Ca(2+) signal is reduced following group I/II metabotropic receptor (mGluR) blockade, the duration is sustained. Based on previous studies it has been hypothesized that the molecular disassembly in astrocytic endfeet is caused by dystrophin cleavage mediated by Ca(2+) dependent proteases. Using a newly developed genetically encoded Ca(2+) sensor, the present study bolsters this hypothesis by demonstrating long-lasting, enhanced stimulation-evoked Ca(2+) signals in astrocytic endfeet.

References

Oct 1, 1992·Journal of Biochemistry·M YoshidaE Ozawa
Jan 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A FrigeriM Svelto
Aug 15, 2001·American Journal of Physiology. Cell Physiology·K HigashiY Kurachi
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·J D NeelyM E Adams
Sep 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zsolt VajdaSøren Nielsen
Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mahmood Amiry-MoghaddamAnish Bhardwaj
Dec 20, 2003·Nature Reviews. Neuroscience·Mahmood Amiry-Moghaddam, Ole P Ottersen
Jan 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tore EidNihal C de Lanerolle
Aug 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Didrik S FrydenlundMahmood Amiry-Moghaddam
Oct 5, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shinghua DingPhilip G Haydon
Jan 28, 2009·PloS One·Johannes HirrlingerFrank Kirchhoff
Jun 18, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Richard H SmithRobert M Kotin
Jul 10, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Wannan TangRolf Sprengel
Nov 26, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Oana CheverFlorin Amzica
Jun 24, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alexander M B ReevesBaljit S Khakh
Sep 6, 2011·Journal of Alzheimer's Disease : JAD·Jing YangReidun Torp
Oct 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Nadia Nabil Haj-YaseinErlend Arnulf Nagelhus
Mar 2, 2012·Glia·Devin K BinderOle Petter Ottersen
May 26, 2012·Trends in Neurosciences·Adrian WaiteDerek J Blake
Aug 11, 2012·Journal of Neuropathology and Experimental Neurology·Kjell HeuserNihal C de Lanerolle
Oct 5, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jasper AkerboomLoren L Looger
Jan 30, 2013·Neurochemistry International·Peter Bedner, Christian Steinhäuser
Jan 30, 2013·Epilepsy Research·Silje AlvestadOle Petter Ottersen
Feb 22, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mutsuo Nuriya, Masato Yasui
Feb 28, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Zeguang RenMaiken Nedergaard
Sep 12, 2013·Neurochemistry International·Devin K Binder, Monica J Carson
Oct 19, 2013·Physiological Reviews·Erlend A Nagelhus, Ole P Ottersen
Mar 13, 2014·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Vincenzo CrunelliChristian Steinhäuser
Feb 24, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Wannan TangErlend A Nagelhus
Mar 15, 2015·Brain : a Journal of Neurology·Peter BednerChristian Steinhäuser

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Citations

Feb 21, 2016·Trends in Cell Biology·Eiji ShigetomiBaljit S Khakh
Mar 10, 2017·Scientific Reports·Hong-Yu LongBo Xiao
Mar 3, 2019·The Journal of Physiology·Yohei Okubo, Masamitsu Iino
Sep 1, 2018·Cerebral Cortex·Kjell HeuserRune Enger
Nov 11, 2019·Glia·Ljiljana NikolicEtienne Audinat
Aug 2, 2016·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Eiji Shigetomi, Schuichi Koizumi
Dec 29, 2020·Glia·Anthony D Umpierre, Long-Jun Wu
Aug 3, 2021·Frontiers in Cellular Neuroscience·Kjell Heuser, Rune Enger

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Methods Mentioned

BETA
restriction digest
PCR

Software Mentioned

Prism
MATLAB
Fiji ImageJ
Fiji
GraphPad

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