Augmentation of GG2EE macrophage cell line-mediated anti-Candida activity by gamma interferon, tumor necrosis factor, and interleukin-1.

Infection and Immunity
E BlasiF Bistoni

Abstract

The expression of anti-Candida activity in the GG2EE macrophage cell line, generated by immortalization of fresh bone marrow with v-raf and v-myc oncogenes, was studied. GG2EE cells spontaneously inhibited the growth of an agerminative mutant of Candida albicans in vitro. The anti-Candida activity was maximal after 8 h of coculture and was proportional to the effector-to-target ratio. Gamma interferon (IFN-gamma), interleukin-1 (IL-1), and tumor necrosis factor (TNF) all significantly enhanced the anti-Candida activity of GG2EE cells. In contrast, IL-3, IL-4, and colony-stimulating factor 1 were ineffective. The augmentation of anti-Candida activity was not always concomitant with enhancement of phagocytosis, since IFN-gamma and colony-stimulating factor 1, but not IL-1 or TNF, augmented the phagocytic ability of GG2EE cells. Furthermore, the augmentation of anti-Candida activity in GG2EE cells did not correlate with the acquisition of antitumor activity. In fact, none of the cytokines alone were able to induce antitumor activity in GG2EE cells, which, however, could be activated to a tumoricidal stage by IFN-gamma plus heat-killed Listeria monocytogenes. These findings demonstrate that GG2EE cells exhibit spontaneous anti-Cand...Continue Reading

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Citations

Mar 1, 1992·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A VecchiarelliG Abbritti
Apr 27, 2005·FEMS Immunology and Medical Microbiology·Elisabetta BlasiSamuele Peppoloni
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Jan 1, 1994·Biotherapy·B J Kullberg, J W van 't Wout
Jun 1, 1993·International Journal of Dermatology·S P Raychaudhuri, S K Raychaudhuri
Nov 1, 1995·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·N VazquezT J Rogers
Dec 1, 1993·FEMS Immunology and Medical Microbiology·L PitzurraE Blasi
Jul 1, 1991·Infection and Immunity·M T Cantorna, E Balish
Jan 1, 1992·Infection and Immunity·H A HidalgoJ A Mangos
Jun 1, 1997·Microbiology and Molecular Biology Reviews : MMBR·A Vázquez-Torres, E Balish
Mar 1, 1994·Chest·A VecchiarelliF Bistoni

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