Augmentation of human cytotoxic T lymphocytes against autologous tumor by a factor released from human monocytic leukemia cell line

Japanese Journal of Cancer Research : Gann
N MaedaS Fujimoto

Abstract

A human acute monocytic leukemia cell line, THP-1, releases a factor which activates human cytotoxic (killer) T lymphocytes (CTL) against autologous tumor in vitro. The factor, named cytotoxic (killer) T cell activating factor (KAF), is an acidic protein of 70,000 to 100,000 dalton molecular size. Peripheral blood leukocytes from two patients, bearing epithelioid sarcoma or malignant schwannoma, were cultured for 7 days with individual autologous tumor to induce CTL directed to the corresponding tumor. Monocyte-depleted peripheral leukocytes generated lesser CTL activity than the monocyte-containing leukocyte population. However, the KAF was able to replace the monocyte function. The KAF acted at the CTL generation phase as well as the effector phase. The KAF-activated killer cells possessed CD4-8+ surface phenotype. The CTL killed autologous tumor or other unrelated tumor cell lines only when they shared some of the HLA class I antigens. It was also demonstrated that the KAF does not activate killer cells without proper antigenic stimuli, because the KAF-augmented CTL possess specificity against autologous tumor or other HLA-A or -B matched tumor cell lines. The therapeutic applicability of human KAF for anti-tumor CTL therapy...Continue Reading

References

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Citations

Jan 1, 1989·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·G Mathé
Jan 1, 1993·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·H SonobeS Fujimoto
Sep 1, 1993·Asia-Oceania Journal of Obstetrics and Gynaecology·N Maeda, Y Sagara
Mar 16, 1995·International Journal of Cancer. Journal International Du Cancer·M TaniH Terasawa

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