Augmentation of immune responses to SARS coronavirus by a combination of DNA and whole killed virus vaccines

Vaccine
Alexander N ZakhartchoukLorne A Babiuk

Abstract

We studied the immunogenicity of a DNA SARS-vaccine, a whole killed virus, or a whole killed and DNA vaccine combination. The DNA vaccine contained a plasmid encoding the SARS coronavirus (SARS-CoV) S protein under the control of the human CMV promoter and intron A. The whole killed virus vaccine was comprised of SARS-CoV, propagated in Vero-E6 cells, with subsequent beta-propilactone inactivation and formulated with aluminum hydroxide adjuvant. Mice immunized twice with the DNA vaccine and once with the whole killed virus elicited higher antibody responses than mice immunized three times with the DNA vaccine or once with the whole killed virus vaccine. Mice immunized twice with the whole killed virus vaccine elicited higher antibody responses than mice immunized three times with the DNA vaccine or once with the whole killed virus vaccine. However, a combination of the vaccines induced T-helper type 1 (Th1) immune responses while the whole killed virus vaccine induced T helper type 2 (Th2) immune response. These results demonstrate that combination of the DNA vaccine and the whole killed virus vaccine can be used to enhance the magnitude and change the bias of the immune responses to SARS-CoV.

References

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Citations

Jan 15, 2014·Antiviral Research·Chung-ke ChangTai-huang Huang
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Susan R Weiss, Sonia Navas-Martin
Oct 17, 2006·Clinical Microbiology Reviews·Laura Gillim-Ross, Kanta Subbarao
Aug 2, 2007·DNA and Cell Biology·Alexander N ZakhartchoukLorne A Babiuk
Oct 13, 2006·Human Gene Therapy·Margaret A LiuGunilla B Karlsson Hedestam
Jun 23, 2009·Expert Review of Vaccines·Rachel L Roper, Kristina E Rehm
May 15, 2007·Virus Research·Anjeanette RobertsKanta Subbarao
Apr 10, 2007·Virus Research·Luis EnjuanesRalph Baric
Dec 24, 2014·Medical Microbiology and Immunology·Anna D KosinskaMichael Roggendorf
Apr 16, 2015·PLoS Neglected Tropical Diseases·Milton MacielRafael Dhalia
Nov 13, 2013·Nature Reviews. Microbiology·Rachel L GrahamRalph S Baric
Aug 14, 2020·Human Vaccines & Immunotherapeutics·Mohamad Hesam ShahrajabianQi Cheng
Dec 31, 2020·Vaccines·Shilu MathewHadi M Yassine
Dec 21, 2020·Journal of Biomedical Science·Yen-Der LiT-C Wu
Jun 15, 2020·Life Sciences·Satish Chandra PandeyMukesh Samant
Aug 12, 2021·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Paul N LevettMel Krajden

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Methods Mentioned

BETA
electrophoresis
ELISA
electron microscopy

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