Augmentation of myocardial transfection using TerplexDNA: a novel gene delivery system

Gene Therapy
D G AffleckSung Wan Kim

Abstract

Gene therapy is a potential new strategy for the treatment of cardiovascular disease. The most efficacious method of gene delivery remains a key hurdle to effective gene therapy. We present the application of a novel, nonviral gene delivery system (TerplexDNA) to augment myocardial transfection. The hearts of New Zealand white rabbits were injected with reporter genes, luciferase cDNA or beta-galactosidase cDNA, either as naked plasmid DNA or plasmid DNA complexed with stearyl-poly(L-lysine)-low density lipoprotein (TerplexDNA). Three day left heart myocardial cell lysates produced 44571 +/- 8730 RLU (RLU = total light units/mg protein) for the TerplexDNA luciferase rabbits versus 1638 +/- 567 RLU for the naked luciferase rabbits (P = 0.002). Thirty days after injection, myocardial lysates produced 677 +/- 52 RLU for the TerplexDNA luciferase hearts versus 18 +/- 3 RLU for the naked luciferase hearts (P = 0.002). Histologic analysis of the hearts transfected with beta-galactosidase showed that TerplexDNA increased the area and depth of transfection compared with the naked plasmid DNA alone. The hearts of Sprague-Dawley rats were injected in a similar fashion and analyzed at 1, 3, 5, 10, 15, 25 and 30 days after injection. The n...Continue Reading

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Citations

Nov 26, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·David A BullSun Wan Kim
Nov 26, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Maureen D BrownIjeoma F Uchegbu
Jun 8, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·L YuS Wan Kim
Jan 5, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jae-Woon NahSung Wan Kim
Sep 3, 2002·Advanced Drug Delivery Reviews·Thomas MerdanThomas Kissel
Mar 10, 2006·Nature Biotechnology·Ascher Shmulewitz, Robert Langer
Sep 1, 2003·Expert Review of Neurotherapeutics·Gustavo PradillaHenry Brem
Mar 26, 2011·Expert Opinion on Biological Therapy·Rabea HinkelChristian Kupatt
Nov 4, 2015·Advanced Drug Delivery Reviews·Pei Yun TeoYi Yan Yang
Jul 6, 2004·Journal of Pharmaceutical Sciences·Jie WenKam W Leong
Jul 31, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Young-Wook WonSung Wan Kim
Jan 19, 2011·Advanced Drug Delivery Reviews·Hyun Ah KimMinhyung Lee
Jun 29, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Youngsook LeeSung Wan Kim
Nov 17, 2007·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·H EliyahuA J Domb
Oct 2, 2003·Nature Biotechnology·David A LaVanRobert Langer
Apr 2, 2004·Nature·Robert Langer, David A Tirrell
Aug 31, 2011·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Zhi-Yi ChenLiang-Ping Luo
Jul 7, 2001·Science·R Langer
Apr 5, 2012·Cold Spring Harbor Protocols·Sung Wan Kim
May 3, 2012·Cold Spring Harbor Protocols·Sung Wan Kim

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