Augmentation of therapeutic angiogenesis using genetically modified human endothelial progenitor cells with altered glycogen synthase kinase-3beta activity

The Journal of Biological Chemistry
Jin-Ho ChoiHyo-Soo Kim

Abstract

Previously we reported that inhibition of glycogen synthase kinase-3beta (GSK3beta), a key regulator in many intracellular signaling pathways, enhances the survival and migration of vascular endothelial cells. Here we investigated the effect of inhibition of GSK3beta activity on the angiogenic function of endothelial progenitor cell (EPC) and demonstrated a new therapeutic angiogenesis strategy using genetically modified EPC. As we previously reported, two biologically distinct types of EPC, spindle-shaped "early EPC" and cobblestone-shaped "late EPC" could be cultivated from human peripheral blood. Catalytically inactive GSK3beta gene was transduced into both EPC. Inhibition of GSK3beta signaling pathway led to increased nuclear translocation of beta-catenin and increased secretion of angiogenic cytokines (vascular endothelial growth factor and interleukin-8). It enhanced the survival and proliferation of early EPC, whereas it promoted the survival and differentiation of late EPC. Transplantation of either of these genetically modified EPC into the ischemic hind limb model of athymic nude mouse significantly improved blood flow, limb salvage, and tissue capillary density compared with nontransduced EPC. Inhibition of GSK3beta ...Continue Reading

References

Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·M DelcommenneS Dedhar
Nov 24, 1999·Molecular and Cellular Biology·S E RossO A MacDougald
Jan 5, 2000·The Journal of Clinical Investigation·Y LinR P Hebbel
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·C KalkaT Asahara
Jun 7, 2000·The Journal of Clinical Investigation·T MuroharaT Imaizumi
Jul 25, 2000·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·C IkpeazuS J Brandt
Jul 7, 2001·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·C G Eberhart, P Argani
Sep 11, 2001·Biochemical and Biophysical Research Communications·A SodhiJ S Gutkind
Feb 28, 2002·Circulation Research·Xiaohong WangJennifer L Hall
Mar 14, 2002·Biochemical and Biophysical Research Communications·Cornelia DietrichFranz Oesch
Apr 23, 2002·The Journal of Biological Chemistry·Thilo HagenAlastair D Reith
Aug 9, 2002·The Journal of Biological Chemistry·Hyo-Soo KimKenneth Walsh
Aug 30, 2002·The Journal of Biological Chemistry·Laurence LévyMarie Annick Buendia
Sep 5, 2002·Circulation·David SimperNoel M Caplice
Feb 14, 2003·The New England Journal of Medicine·Jonathan M HillToren Finkel
Apr 30, 2003·Nature·Tannishtha ReyaIrving L Weissman
Jul 10, 2003·The Journal of Biological Chemistry·Masaki MogiKenneth Walsh
Dec 31, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Jin HurYoung-Bae Park

❮ Previous
Next ❯

Citations

Aug 21, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Xabier L ArangurenAernout Luttun
Jun 19, 2013·Annals of Hematology·Daniel BarthelmesWeiyong Shen
Nov 11, 2010·Current Treatment Options in Cardiovascular Medicine·David A D'Alessandro, Robert E Michler
Jan 19, 2007·Nature Clinical Practice. Cardiovascular Medicine·Florian H SeegerStefanie Dimmeler
Aug 18, 2010·Antioxidants & Redox Signaling·Felix Fleissner, Thomas Thum
Mar 22, 2013·BioResearch Open Access·Vahid RazbanAlireza Khoshdel
Aug 15, 2012·Tissue Engineering. Part C, Methods·Praveen K SobhanM Radhakrishna Pillai
Apr 1, 2008·Annals of Surgery·Ralf W SprengersMarianne C Verhaar
Jan 18, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Radwan N SafaDouglas B Sawyer
Mar 20, 2010·Journal of Angiogenesis Research·Aurangzeb SiddiqueChetan Varma
Mar 27, 2007·Circulation Journal : Official Journal of the Japanese Circulation Society·Jin-Ho ChoiEun-Seok Jeon
May 19, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Beata Kieć-WilkAldona Dembińska-Kieć
Nov 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Claudio NapoliLouis J Ignarro
Sep 9, 2008·Expert Opinion on Biological Therapy·Serap GurWayne J G Hellstrom
Aug 9, 2011·Experimental Diabetes Research·Stella BernardiPaola Secchiero
Sep 20, 2011·Blood·Yushan Zhang, Yehia Daaka
Feb 27, 2007·Biologicals : Journal of the International Association of Biological Standardization·Akiko Ishii-WatabeToru Kawanishi
Dec 5, 2006·The Journal of Thoracic and Cardiovascular Surgery·Hideaki YamabiJohn G Coles
Feb 23, 2017·Journal of Molecular and Cellular Cardiology·Francisco M VegaMª Carmen Duran-Ruiz
Jun 30, 2007·The Journal of Thoracic and Cardiovascular Surgery·Sara ArabJohn G Coles
Dec 8, 2017·Regenerative Medicine·Khawaja Husnain HaiderMateq Ali Al-Reshidi
Mar 20, 2010·Circulation Research·Yagna P R Jarajapu, Maria B Grant
Oct 15, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Mejeong LeeToyoaki Murohara
Apr 2, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Hyun-Jai ChoHyo-Soo Kim
Oct 3, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Anne M GoodwinPatricia A D'Amore
Jun 14, 2008·Virchows Archiv : an International Journal of Pathology·Yoko Abe-YoshioShigeru Kohno
Nov 6, 2008·Journal of Biomedical Materials Research. Part a·Zhenhua ZhouChuhong Zhu
Nov 3, 2005·Journal of Biomedical Materials Research. Part a·Seung-Woo ChoByung-Soo Kim

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.