Augmentation of vaccenate production and suppression of vaccenate biohydrogenation in cultures of mixed ruminal microbes

Journal of Dairy Science
S FukudaTsuneo Hino

Abstract

To increase ruminal outflow of trans-vaccenic acid (t-VA), a new strain of Butyrivibrio fibrisolvens (MDT-10) was isolated that has a great ability to hydrogenate linoleic acid (LA) to t-VA. When strain MDT-10 was added to the batch cultures of mixed ruminal microbes (1% of the total number of viable ruminal bacteria), LA conversion to t-VA increased greatly; after 3 h, t-VA levels were > 4-fold higher than the control. By 10 h, all of the t-VA was hydrogenated to stearic acid. However, when a new strain of Bifidobacterium adolescentis (HF-11), which has a high capacity for incorporation of t-VA, was added in conjunction with MDT-10 (1% of the total number of ruminal bacteria), t-VA levels after 10 h were 6 times higher than with MDT-10 alone. These results suggest that t-VA produced by MDT-10 was incorporated into HF-11 cells, resulting in protection of t-VA from t-VA-hydrogenating microbes. Similar results were obtained in a continuous culture of mixed ruminal microbes in which addition of HF-11 simultaneously with MDT-10 increased the amount of t-VA in the effluent 2.5-fold. Both MDT-10 and HF-11 appeared to grow readily in the presence of mixed ruminal microbes. Sixty-two percent of t-VA incorporated by HF-11 was present in...Continue Reading

References

Aug 1, 1993·Journal of Dairy Science·T HinoS Kumazawa
Aug 16, 1997·Biochimica Et Biophysica Acta·M C BarberR G Vernon
Aug 29, 1998·Journal of Applied Microbiology·J JiangR Fondén
Apr 5, 2001·Journal of Dairy Science·P Y ChouinardD E Bauman
May 29, 2002·The Journal of Nutritional Biochemistry·Benjamin A. CorlDale E. Bauman
Dec 25, 2002·The Journal of Veterinary Medical Science·Shinji FukudaTsuneo Hino
Mar 11, 2003·Annual Review of Nutrition·Dale E Bauman, J Mikko Griinari
May 26, 2004·The American Journal of Clinical Nutrition·Michael W Pariza
May 26, 2004·The American Journal of Clinical Nutrition·Yanwen Wang, Peter J H Jones
Nov 4, 2004·Progress in Lipid Research·Klaus W J WahleDino Rotondo
Jun 9, 2005·The Journal of General and Applied Microbiology·Shinji FukudaTsuneo Hino

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