Augmented nitric oxide production and up-regulation of endothelial nitric oxide synthase during cecal ligation and perforation

Nitric Oxide : Biology and Chemistry
Alice V AraújoLusiane M Bendhack

Abstract

Nitric oxide (NO) has been pointed out as being the main mediator involved in the hypotension and tissue injury taking place during sepsis. This study aimed to investigate the cellular mechanisms implicated in the acetylcholine (ACh)-induced relaxation detected in aortic rings isolated from rats submitted to cecal ligation and perforation (CLP group), 6h post-CLP. The mean arterial pressure was recorded, and the concentration-effect curves for ACh were constructed for endothelium-intact aortic rings in the absence (control) or after incubation with one of the following NO synthase inhibitors: L-NAME (non-selective), L-NNA (more selective for eNOS), 7-nitroindazole (more selective for nNOS), or 1400W (selective for iNOS). The NO concentration was determined by using confocal microscopy. The protein expression of the NOS isoforms was quantified by Western blot analysis. The prostacyclin concentration was indirectly analyzed on the basis of 6-keto-prostaglandin F(1α) (6-keto-PGF(1α)) levels measured by enzyme immunoassay. There were no differences between Sham- and CLP-operated rats in terms of the relaxation induced by acetylcholine. However, the NOS inhibitors reduced this relaxation in both groups, but this effect remained more...Continue Reading

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Citations

Aug 9, 2015·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Karin ScheschowitschJamil Assreuy
Sep 19, 2012·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Mara R N CelesMarcos A Rossi
Mar 22, 2020·International Journal of Molecular Sciences·Itaynara L DutraAfonso G Abreu
Feb 2, 2021·Frontiers in Physiology·Daniela Esteves Ferreira Dos Reis CostaDaniella Bonaventura

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