Augmented Pla2g4c/Ptgs2/Hpgds axis in bronchial smooth muscle tissues of experimental asthma

PloS One
Yoshihiko ChibaHiroyasu Sakai

Abstract

Augmented smooth muscle contractility of the airways is one of the causes of airway hyperresponsiveness in asthmatics. However, the mechanism of the altered properties of airway smooth muscle cells is not well understood. To identify differentially expressed genes (DEGs) related to the bronchial smooth muscle (BSM) hyper-contractility in a murine asthma model. The ovalbumin (OA)-sensitized mice were repeatedly challenged with aerosolized OA to induce asthmatic reaction. Transcriptomic profiles were generated by microarray analysis of BSM tissues from the OA-challenged and control animals, and KEGG (Kyoto Encyclopedia of Genes and Genomes) Pathway Analysis was applied. Tension study showed a BSM hyperresponsiveness to acetylcholine (ACh) in the OA-challenged mice. A total of 770 genes were differentially expressed between the OA-challenged and control animals. Pathway analysis showed a significant change in arachidonic acid (AA) metabolism pathway in BSM tissues of the OA-challenged mice. Validation of DEGs by quantitative RT-PCR showed a significant increase in PLA2 group 4c (Pla2g4c)/COX-2 (Ptgs2)/PGD2 synthase 2 (Hpgds) axis. PGD2 level in bronchoalveolar fluids of the OA-challenged mice was significantly increased. A 24-h in...Continue Reading

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Citations

Nov 7, 2019·Current Opinion in Allergy and Clinical Immunology·Xuesong ChenEvan Li
Sep 3, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yoshihiko ChibaSeiji Shioda
Nov 3, 2020·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Yoshihiko Chiba
Oct 31, 2020·International Journal of Molecular Sciences·Yoshihiko ChibaHiroyasu Sakai
Apr 5, 2021·Respiratory Physiology & Neurobiology·Yoshihiko ChibaHiroyasu Sakai
Jul 21, 2021·Molecular Aspects of Medicine·Shengjie XuJoseph Jude

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Methods Mentioned

BETA
Feature Extraction
PCR
lavage
bronchoalveolar lavage
bronchoalveolar
ELISA
enzyme-linked immunosorbent assay
chip
electrophoresis

Software Mentioned

R
LabSolutions
Agilent Feature Extraction Protocol
Gene Set Enrichment Analysis
StatView
GeneCodis
Agilent Feature Extraction

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