Augmented renal vascular nNOS and renin protein expression in angiotensin type 1 receptor null mice

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Sungmi Park, Lisa M Harrison-Bernard

Abstract

The present study was performed to determine the influence of absence of angiotensin type 1A (AT(1A)) and/or AT(1B) receptor feedback regulation of kidney neuronal nitric oxide synthase (nNOS) and renin protein expression. Kidneys were harvested from wild-type (WT), AT(1A)(-/-), AT(1B)(-/-), and AT(1A)(-/-)AT(1B)(-/-) mice and immunostained for nNOS and renin protein localization. AT(1A)(-/-) and AT(1A)(-/-)AT(1B)(-/-) kidneys demonstrated an increase in the percentage of glomeruli with nNOS-positive afferent and interlobular arterioles compared with WT mice. Density of vascular nNOS immunostaining was 20-fold higher in kidneys of AT(1A)(-/-) and AT(1A)(-/-)AT(1B)(-/-) compared with WT mice. Density of macula densa nNOS immunostaining was 7-fold higher in AT(1A)(-/-)AT(1B)(-/-) than in WT mice. Percent of glomeruli positive for juxtaglomerular (JG) cell renin was 3-fold higher, whereas the density of JG cell renin immunostaining was 15-fold higher in kidneys of AT(1A)(-/-) and AT(1A)(-/-)AT(1B)(-/-) compared with WT mice. Kidneys of AT(1A)(-/-) and AT(1A)(-/-)AT(1B)(-/-) mice displayed recruitment of renin protein expression along afferent and interlobular arterioles. Absence of AT(1) receptor signaling resulted in enhanced nNO...Continue Reading

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Apr 6, 2007·American Journal of Physiology. Renal Physiology·Sungmi ParkLisa M Harrison-Bernard
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Citations

Oct 23, 2009·American Journal of Physiology. Renal Physiology·Sungmi ParkLisa M Harrison-Bernard
Jan 20, 2012·Kidney International·Jurgen Schnermann, Josephine P Briggs
Feb 5, 2015·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Yanling ZhangTingting Chen
Jan 8, 2009·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Zsolt Razga, Jens Randel Nyengaard
Feb 8, 2018·American Journal of Physiology. Renal Physiology·Sungmi ParkLisa M Harrison-Bernard
Feb 10, 2017·American Journal of Physiology. Renal Physiology·Sebastien A RiderJohn J Mullins
Aug 28, 2009·American Journal of Physiology. Renal Physiology·Katharina MachuraArmin Kurtz
Dec 25, 2019·Physiological Reports·Benjamin J BivonaLisa M Harrison-Bernard

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