Abstract
Sphingosylphosphorylcholine (SPC) is a vasoconstricting lysosphingolipid, and the RhoA/Rho-kinase pathway plays an important role in SPC-induced contraction. Since RhoA/Rho-kinase-mediated signaling is involved in the generation and/or maintenance of hypertension, we compared the effect of SPC on the contractility of endothelium-denuded small mesenteric arteries in spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKY). Fura-2 Ca2+ signals, contractile responses, and phosphorylation of 20-kDa myosin light chains (MLC20) were measured. Ten microM SPC induced a gradual and sustained vasoconstriction, which was greater in arteries of the SHR (82.5 +/- 4.3%, n=9) than in those of the WKY (26.7 +/- 4.5%, n=10). In Ca2+-free media, SPC gradually increased vascular tone in the SHR, but caused little vasoconstriction in the WKY. In the SHR and WKY, SPC evoked a greater vasoconstriction than did high K+ depolarization at a given Ca2+ ratio, and the Ca2+ ratio-tension curve induced by SPC was significantly shifted to the left compared with that induced by high K+ depolarization. However, the magnitude of shift to the left was greater in the SHR than in the WKY. The Rho-kinase inhibitor Y-27632 significantly inhibited SPC-induc...Continue Reading
References
Oct 1, 1994·The Journal of Clinical Investigation·S SatohG Pfitzer
Aug 1, 1995·The Journal of Membrane Biology·S Spiegel, S Milstien
Jul 12, 1996·Science·K KimuraK Kaibuchi
Sep 1, 1996·British Journal of Pharmacology·P E Jensen
Nov 14, 1997·Nature·M UehataS Narumiya
Nov 14, 1998·The American Journal of Physiology·T Y Chin, S H Chueh
Mar 19, 1999·Circulation Research·R M TouyzE L Schiffrin
Aug 22, 2000·British Journal of Pharmacology·A BischoffM C Michel
Oct 6, 2000·FEBS Letters·N Todoroki-IkedaS Kobayashi
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Y MukaiA Takeshita
Apr 18, 2001·Biochemistry·Y A HannunK M Argraves
Sep 6, 2001·Naunyn-Schmiedeberg's Archives of Pharmacology·A BischoffM C Michel
Dec 26, 2001·Hypertension·A MasumotoA Takeshita
Feb 6, 2002·Cardiovascular Research·Dong Soo YeonYoung Ho Lee
Jul 27, 2002·Circulation Research·Satoshi ShiraoSei Kobayashi
Jul 27, 2002·Circulation Research·Frederic CoussinGraeme F Nixon
Oct 24, 2002·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Nina Wettschureck, Stefan Offermanns
Nov 16, 2002·Circulation Research·Fumiaki NakaoMasunori Matsuzaki
Jan 11, 2003·British Journal of Pharmacology·Christoph AltmannMartin C Michel
Mar 29, 2003·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Masahisa Asano, Yukiko Nomura
Oct 27, 2004·British Journal of Pharmacology·Astrid E AlewijnseMartin C Michel
Apr 19, 2005·Journal of Hypertension·Carmine SavoiaRhian M Touyz
Jun 14, 2005·Cardiovascular Research·Gavin D ThomasJeremy P T Ward
Oct 19, 2005·Naunyn-Schmiedeberg's Archives of Pharmacology·Gil-Jin JangYoung-Ho Lee
Citations
Mar 30, 2006·Naunyn-Schmiedeberg's Archives of Pharmacology·Denise G Hemmings
Oct 14, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·Hye Young KimIn Kyeom Kim
Apr 22, 2010·Biochemical and Biophysical Research Communications·Hae-Ahm LeeIn Kyeom Kim
Mar 30, 2007·Acta Paediatrica. Supplement·Martin C MichelStephan L M Peters
Jan 20, 2015·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Young-Hwan KimSeungsoo Chung
Feb 17, 2009·Cardiovascular Research·Soo-Kyoung ChoiYoung-Ho Lee
Mar 12, 2010·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Enyue YangIn Kyeom Kim