Augmenter of liver regeneration attenuates inflammation of renal ischemia/reperfusion injury through the NF-kappa B pathway in rats

International Urology and Nephrology
Ruyu YanHui Guo

Abstract

The effects of augmenter of liver regeneration (ALR) on the acute kidney injury (AKI) rats were investigated by measuring the inflammatory response associated with transcription factor nuclear factory (NF-κB) pathway. The model of AKI rats was established by occluded the renal pedicles for 60 min and then released. After that, animals were treated with ALR (100 or 200 μg/kg). All rats were killed at different time points (24, 48, 72 h). Renal function and kidney histological changes were measured. The apoptosis of tubular cells was evaluated by TdT-mediated dUTP nick end labeling assay. Cytokines and chemokines were assessed by immunohistochemistry, enzyme-linked immunosorbent assay and real-time polymerase chain reaction (RT-PCR). The NF-κB p65 protein was analyzed by immunohistochemistry and RT-PCR, respectively. Ischemia reperfusion induced tubular cells necrosis and apoptosis, and ALR can significantly reduce this damages. The productions of MCP-1, IL-1β and IL-6 were lower in the group of ALR treatment, especially in the high-dose group. The inflammatory infiltrates were lower in the rats with administration of ALR. ALR mediated the level of cytokines and chemokines through inhibited the activation of NF-κB. ALR can improv...Continue Reading

References

Jan 1, 1996·Annual Review of Immunology·A S Baldwin
Mar 30, 2002·Journal of the American College of Cardiology·Naoya MaekawaMitsuru Seishima
Nov 5, 2002·The American Journal of Pathology·Atsushi KatoAlex B Lentsch
Jul 16, 2004·Kidney International·Joseph V Bonventre, Anna Zuk
Feb 1, 2008·Journal of the American Society of Nephrology : JASN·Dmitry N GrigoryevHamid Rabb
Sep 20, 2008·Nephron. Experimental Nephrology·Gilbert R KinseyMark D Okusa
Mar 25, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Xiao-hui LiaoHui Guo
May 12, 2010·Cold Spring Harbor Perspectives in Biology·Toby Lawrence
Feb 4, 2011·BMC Medicine·Raj MunshiJonathan Himmelfarb
Jun 26, 2012·Current Pharmaceutical Design·Chiara Gasparini, Marc Feldmann
Jul 11, 2012·Fibrogenesis & Tissue Repair·Chandrashekhar R Gandhi
Jun 4, 2013·Lancet·Norbert H LameireRaymond Vanholder
Dec 1, 2007·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·Bernhard DorweilerChristian F Vahl

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Citations

Sep 24, 2015·Canadian Journal of Physiology and Pharmacology·Wei ChenYong Jin
Jan 14, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Michael A NalesnikThomas E Starzl
Nov 26, 2015·World Journal of Gastroenterology : WJG·Guang-Hua TangYi-Lei Mao
May 4, 2017·Apoptosis : an International Journal on Programmed Cell Death·Tao PuQi Liu
Sep 28, 2018·Apoptosis : an International Journal on Programmed Cell Death·Li-Li HuangXiao-Hui Liao
Nov 6, 2020·Dose-response : a Publication of International Hormesis Society·Tingting ZhangRensheng Wang

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