Augmenting the antibiofilm efficacy of advanced noninvasive light activated disinfection with emulsified oxidizer and oxygen carrier

Journal of Endodontics
Saji George, Anil Kishen

Abstract

In this study, we tested the hypothesis that the inclusion of an oxidizer and oxygen carrier in the photosensitization formulation would facilitate comprehensive disinfection of matured endodontic biofilm by light-activated disinfection (LAD). Photosensitizing formulations containing methylene blue (MB) and an oxygen carrier alone (perfluorodecahydronaphthalene) (PF1) or in combination with oxidizer (H(2)O(2)) (PF2) or their emulsions formed with triton-X100 (Bio-Rad Laboratories, Hercules, CA) in different proportions (PF3 and PF4) were tested for photochemical properties and damage to the biofilm structure using confocal laser scanning microscopy. Conventional chemomechanical preparation, LAD using MB in water, and LAD using MB in emulsion (PF4) were also conducted on 10-week-old Enterococcus faecalis biofilm within root canals. MB in emulsion (PF4) was overall the most effective photosensitizer formulation for photooxidation, generation of singlet oxygen (p = 0.001), and in disinfecting biofilm bacteria. Advanced noninvasive LAD using a photosensitizer formulation containing oxidizer and oxygen carrier disrupted the biofilm matrix and facilitated comprehensive inactivation of biofilm bacteria. This modified photosensitizer f...Continue Reading

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Citations

Sep 24, 2010·Photochemistry and Photobiology·Anil KishenMichael R Hamblin
Dec 23, 2011·International Journal of Dentistry·Sushma Jaju, Prashant P Jaju
Mar 2, 2011·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Gianluca GambariniLuca Testarelli
Oct 22, 2013·Journal of Endodontics·Evren OkTuba Gok
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Jul 2, 2009·Journal of Endodontics·Bing FanJames L Gutmann
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Nov 3, 2011·Photochemistry and Photobiology·Annie Shrestha, Anil Kishen
Nov 19, 2014·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Shipra SinghSashi Prabha Tyagi
Aug 21, 2015·Nigerian Journal of Clinical Practice·Ö TuncayS Demirbuga
Jan 18, 2011·Journal of Endodontics·Raymond NgNikolaos S Soukos
Feb 27, 2015·Photomedicine and Laser Surgery·Alessandra Cesar TrindadeJoão Batista Blessmann Weber
May 25, 2016·Journal of Conservative Dentistry : JCD·Anil KishenMadhu K Nair
Jun 23, 2016·Journal of Lasers in Medical Sciences·Nasim ChiniforushAbbas Bahador
Aug 21, 2010·International Endodontic Journal·M H Upadya, A Kishen
Dec 15, 2018·International Endodontic Journal·G PlotinoM Mercade
Jul 13, 2018·Dentistry Journal·Islam A Abdelaziz Ali, Prasanna Neelakantan
Mar 6, 2019·Restorative Dentistry & Endodontics·Yeon-Jee YooKee-Yeon Kum
Jun 18, 2017·The Journal of Contemporary Dental Practice·Zahed MohammadiJun-Ichiro Kinoshita

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