Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission

Molecular Biology of the Cell
Ryota UeharaGohta Goshima

Abstract

During anaphase, distinct populations of microtubules (MTs) form by either centrosome-dependent or augmin-dependent nucleation. It remains largely unknown whether these different MT populations contribute distinct functions to cytokinesis. Here we show that augmin-dependent MTs are required for the progression of both furrow ingression and abscission. Augmin depletion reduced the accumulation of anillin, a contractile ring regulator at the cell equator, yet centrosomal MTs were sufficient to mediate RhoA activation at the furrow. This defect in contractile ring organization, combined with incomplete spindle pole separation during anaphase, led to impaired furrow ingression. During the late stages of cytokinesis, astral MTs formed bundles in the intercellular bridge, but these failed to assemble a focused midbody structure and did not establish tight linkage to the plasma membrane, resulting in furrow regression. Thus augmin-dependent acentrosomal MTs and centrosomal MTs contribute to nonredundant targeting mechanisms of different cytokinesis factors, which are required for the formation of a functional contractile ring and midbody.

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Citations

Sep 11, 2017·FEBS Letters·Shota HirumaRyota Uehara
Oct 13, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Corina DekrakerCraig A Mandato
Jun 5, 2020·The Journal of Cell Biology·Bernardo Chapa-Y-LazoMasanori Mishima
Aug 25, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alicia G GubiedaJosana Rodriguez
Jan 12, 2017·Protoplasma·Vadym SulimenkoPavel Dráber
Jul 26, 2019·The Journal of Cell Biology·Vikash Verma, Thomas J Maresca
Sep 19, 2019·Journal of Cell Science·Kruno VukušićIva M Tolić
May 2, 2018·The Journal of Cell Biology·Kan YaguchiRyota Uehara
Apr 15, 2021·Seminars in Cell & Developmental Biology·Kruno Vukušić, Iva M Tolić
Jun 5, 2021·Nature Chemical Biology·Nandini ManiRadhika Subramanian

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Methods Mentioned

BETA
GTPase
Fluorescence recovery after photobleaching
Fluorescence
transmission electron microscopy
transfection
PCR

Software Mentioned

ImageJ

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