AuNPs modified, disposable, ITO based biosensor: Early diagnosis of heat shock protein 70

Biosensors & Bioelectronics
Münteha Nur Sonuç KaraboğaMustafa Kemal Sezgintürk

Abstract

This paper describes a novel, simple, and disposable immunosensor based on indium-tin oxide (ITO) sheets modified with gold nanoparticles to sensitively analyze heat shock protein 70 (HSP70), a potential biomarker that could be evaluated in diagnosis of some carcinomas. Disposable ITO coated Polyethylene terephthalate (PET) electrodes were used and modified with gold nanoparticles in order to construct the biosensors. Optimization and characterization steps were analyzed by electrochemical techniques such as electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Surface morphology of the biosensor was also identified by electrochemical methods, scanning electron microscopy (SEM), and atomic force microscopy (AFM). To interpret binding characterization of HSP70 to anti-HSP70 single frequency impedance method was successfully operated. Moreover, the proposed HSP70 immunosensor acquired good stability, repeatability, and reproducibility. Ultimately, proposed biosensor was introduced to real human serum samples to determine HSP70 sensitively and accurately.

References

Mar 9, 2002·Science·F Ulrich Hartl, Manajit Hayer-Hartl
Dec 25, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Makoto ChumaSetsuo Hirohashi
Mar 17, 2005·Cellular and Molecular Life Sciences : CMLS·M P Mayer, B Bukau
Jul 26, 2005·Cell Stress & Chaperones·Daniel R Ciocca, Stuart K Calderwood
Feb 18, 2006·Trends in Biochemical Sciences·Stuart K CalderwoodDaniel R Ciocca
Aug 10, 2007·Molecular and Cellular Probes·J C Y KahC J R Sheppard
Dec 14, 2011·Pancreas·Sudhir K DuttaNipun B Merchant
Apr 9, 2013·Carcinogenesis·Maureen E Murphy
Aug 4, 2000·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·A N ShipwayI Willner

❮ Previous
Next ❯

Citations

Nov 8, 2016·Biosensors & Bioelectronics·Masumi Iijima, Shun'ichi Kuroda
Sep 28, 2017·Pharmaceutics·Ayesha ManzurClare Hoskins
Jul 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Baozhen WangJun-Ichi Anzai
Feb 24, 2020·Seminars in Cancer Biology·Christopher WoodmanCornelia M Wilson
Dec 31, 2020·ACS Biomaterials Science & Engineering·Marta NyczDorota Genowefa Pijanowska
Jan 21, 2017·Analytical Chemistry·Wei WenYuehe Lin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosensors for Cancer Detection

Biosensors are devices that are designed to detect a specific biological analyte by essentially converting a biological entity (ie, protein, DNA, RNA) into an electrical signal that can be detected and analyzed. The use of biosensors in cancer detection and monitoring holds vast potential. Biosensors can be designed to detect emerging cancer biomarkers and to determine drug effectiveness at various target sites. Biosensor technology has the potential to provide fast and accurate detection, reliable imaging of cancer cells, and monitoring of angiogenesis and cancer metastasis, and the ability to determine the effectiveness of anticancer chemotherapy agents.