AuNPs/CNOs/SWCNTs/chitosan-nanocomposite modified electrochemical sensor for the label-free detection of carcinoembryonic antigen

Biosensors & Bioelectronics
Mohammad RizwanMinhaz Uddin Ahmed

Abstract

In this work, a nanocomposite of gold nanoparticles (AuNPs), carbon nano-onions (CNOs), single-walled carbon nanotubes (SWCNTs) and chitosan (CS) (AuNPs/CNOs/SWCNTs/CS) was prepared for the development of highly sensitive electrochemical immunosensor for the detection of carcinoembryonic antigen (CEA), clinical tumor marker. Firstly, layer-by-layer fabrication of the CEA-immunosensors was studied using cyclic voltammetry (CV) and square wave voltammetry (SWV). By combining the advantages of large surface area and electronic properties of AuNPs, CNOs, SWCNTs, and film forming properties of CS, AuNPs/CNOs/SWCNTs/CS-nanocomposite-modified glassy carbon electrode showed a 200% increase in effective surface area and electronic conductivity. The calibration plot gave a negative linear relationship between log[concentration] of CEA and electrical current with a correlation coefficient of 0.9875. The CEA-immunosensor demonstrated a wide linear detection range of 100 fg mL-1 to 400 ng mL-1 with a low detection limit of 100 fg mL-1. In addition to high sensitivity, reproducibility and large stability, CEA-immunosensor provided an excellent selectivity and resistant-to-interference in the presence of other antigens in serum and hence a po...Continue Reading

Citations

May 14, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nura Fazira Noor AzamMinhaz Uddin Ahmed
Jul 24, 2020·Sensors·Veronika GajdosovaJan Tkac
Jan 20, 2021·Analytica Chimica Acta·Thangapandi KalyaniSaikat Kumar Jana
Oct 13, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Chitra Padmakumari KurupMinhaz Uddin Ahmed

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