Aurora A activation in mitosis promoted by BuGZ

The Journal of Cell Biology
Yuejia HuangYixian Zheng

Abstract

Protein phase separation or coacervation has emerged as a potential mechanism to regulate biological functions. We have shown that coacervation of a mostly unstructured protein, BuGZ, promotes assembly of spindle and its matrix. BuGZ in the spindle matrix binds and concentrates tubulin to promote microtubule (MT) assembly. It remains unclear, however, whether BuGZ could regulate additional proteins to promote spindle assembly. In this study, we report that BuGZ promotes Aurora A (AurA) activation in vitro. Depletion of BuGZ in cells reduces the amount of phosphorylated AurA on spindle MTs. BuGZ also enhances MCAK phosphorylation. The two zinc fingers in BuGZ directly bind to the kinase domain of AurA, which allows AurA to incorporate into the coacervates formed by BuGZ in vitro. Importantly, mutant BuGZ that disrupts the coacervation activity in vitro fails to promote AurA phosphorylation in Xenopus laevis egg extracts. These results suggest that BuGZ coacervation promotes AurA activation in mitosis.

References

Aug 15, 2002·The Journal of Cell Biology·Thomas A KuferErich A Nigg
Nov 8, 2002·Proceedings of the National Academy of Sciences of the United States of America·Laurie E LittlepageJoan V Ruderman
Feb 11, 2003·Nature Cell Biology·Ming-Ying TsaiYixian Zheng
Apr 18, 2003·Current Biology : CB·Patrick A EyersJames L Maller
Oct 29, 2003·Molecular Cell·Richard BaylissElena Conti
Dec 6, 2006·The Journal of Cell Biology·Davide MarenduzzoPeter R Cook
Apr 25, 2008·Molecular Biology of the Cell·Xin ZhangClaire E Walczak
Aug 30, 2008·The EMBO Journal·Teresa SardonIsabelle Vernos
Feb 10, 2009·Nature Cell Biology·Li MaYixian Zheng
Sep 10, 2010·The Journal of Biological Chemistry·Benjamin GoodmanYixian Zheng
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Vladimir JoukovDavid M Livingston
Aug 7, 2012·Cellular and Molecular Life Sciences : CMLS·Anna S NikonovaErica A Golemis
Jan 28, 2014·Developmental Cell·Chad M ToledoPatrick J Paddison
Apr 29, 2015·The Journal of Cell Biology·Yihan WanYixian Zheng
Aug 5, 2015·The Journal of Cell Biology·Jingyan FuChuanmao Zhang
Oct 7, 2015·The Journal of Cell Biology·Hao JiangYixian Zheng
Dec 30, 2015·Cell Cycle·Nicholas R LimDominic C H Ng

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Citations

Jan 18, 2019·Biomolecules·Laura Magnaghi-JaulinRégis Giet
Dec 22, 2017·The Journal of Cell Biology·Jeffrey B Woodruff
Oct 23, 2019·The Journal of Cell Biology·Johannes BaumgartJan Brugués
Jul 24, 2020·The Journal of Biological Chemistry·Xing LiuXuebiao Yao
Jun 18, 2020·Science China. Life Sciences·Hong ZhangXueliang Zhu
Nov 16, 2018·Essays in Biochemistry·Lori Borgal, James G Wakefield
Aug 16, 2018·Science Signaling·Vladimir Joukov, Arcangela De Nicolo
May 31, 2018·Molecular Medicine Reports·Xiao-Fei DingYing-Liang Wu
Aug 21, 2020·The Journal of Biological Chemistry·Hazheen K ShirnekhiJennifer G DeLuca
Nov 11, 2020·Nature Reviews. Molecular Cell Biology·Andrew S LyonMichael K Rosen
Dec 7, 2020·Cytoskeleton·Joseph Y OngJorge Z Torres
Jan 19, 2021·Current Opinion in Cell Biology·Marta Badia, Benedetta Bolognesi
Feb 22, 2021·Genomics, Proteomics & Bioinformatics·Boyan ShenTingting Li
Apr 10, 2021·The Journal of Cell Biology·Myreille LaroucheVincent Archambault
Aug 30, 2020·Molecular Cell·Joseph Y Ong, Jorge Z Torres
Sep 24, 2021·Journal of Molecular Cell Biology·Manjuan ZhangXuebiao Yao

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Methods Mentioned

BETA
immunoprecipitation
immunoprecipitations
pulldown
pulldowns
pulled
pull down
pulled down

Software Mentioned

Illustrator
Adobe
Photoshop

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