Aurora A-dependent CENP-A phosphorylation at inner centromeres protects bioriented chromosomes against cohesion fatigue

Nature Communications
Grégory Eot-HoullierChristian Jaulin

Abstract

Sustained spindle tension applied to sister centromeres during mitosis eventually leads to uncoordinated loss of sister chromatid cohesion, a phenomenon known as "cohesion fatigue." We report that Aurora A-dependent phosphorylation of serine 7 of the centromere histone variant CENP-A (p-CENP-AS7) protects bioriented chromosomes against cohesion fatigue. Expression of a non-phosphorylatable version of CENP-A (CENP-AS7A) weakens sister chromatid cohesion only when sister centromeres are under tension, providing the first evidence of a regulated mechanism involved in protection against passive cohesion loss. Consistent with this observation, p-CENP-AS7 is detected at the inner centromere where it forms a discrete domain. The depletion or inhibition of Aurora A phenocopies the expression of CENP-AS7A and we show that Aurora A is recruited to centromeres in a Bub1-dependent manner. We propose that Aurora A-dependent phosphorylation of CENP-A at the inner centromere protects chromosomes against tension-induced cohesion fatigue until the last kinetochore is attached to spindle microtubules.

References

Mar 7, 2002·Developmental Cell·Michael D BlowerGary H Karpen
Apr 5, 2002·Molecular Cell·Izabela SumaraJan-Michael Peters
Aug 15, 2002·The Journal of Cell Biology·Thomas A KuferErich A Nigg
Oct 3, 2003·The Journal of Biological Chemistry·Tomotoshi MarumotoHideyuki Saya
Oct 12, 2004·Nature Structural & Molecular Biology·Beth A Sullivan, Gary H Karpen
Jan 5, 2005·Nature Reviews. Cancer·Tomotoshi MarumotoHideyuki Saya
Mar 17, 2006·Nature·Tomoya S KitajimaYoshinori Watanabe
Dec 18, 2007·Nature Cell Biology·Jibak LeeYoshinori Watanabe
Aug 22, 2008·Nature·Yuya YamagishiYoshinori Watanabe
Nov 6, 2009·Annual Review of Genetics·Kim Nasmyth, Christian H Haering
May 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Susana Abreu RibeiroWilliam C Earnshaw
Jun 11, 2011·Current Biology : CB·John R DaumGary J Gorbsky
Feb 24, 2012·Molecular Biology of the Cell·Ewa NiedzialkowskaP Todd Stukenberg
Nov 24, 2012·Nature Reviews. Molecular Cell Biology·Mar CarmenaWilliam C Earnshaw
May 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Damien Goutte-GattatStefan Dimitrov
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Aaron O BaileyDaniel R Foltz
Aug 1, 2013·Proceedings of the National Academy of Sciences of the United States of America·Tomoko NishiyamaJan-Michael Peters
Mar 3, 2015·Developmental Cell·Yohei NiikuraKatsumi Kitagawa
Jul 15, 2015·Current Biology : CB·Anna A YeThomas J Maresca
Nov 26, 2015·Nature Reviews. Molecular Cell Biology·Kara L McKinley, Iain M Cheeseman
Dec 27, 2015·Trends in Biochemical Sciences·Prasad Trivedi, P Todd Stukenberg

❮ Previous
Next ❯

Citations

Jan 18, 2019·Biomolecules·Laura Magnaghi-JaulinRégis Giet
Nov 30, 2018·Molecular Biology of the Cell·Jovana DereticJulie P I Welburn
Nov 13, 2019·The Plant Journal : for Cell and Molecular Biology·Chao FengFangpu Han
Jan 30, 2020·Cell Cycle·M Lienhard SchmitzMarkus Seibert
May 27, 2020·Essays in Biochemistry·Qian Zhang, Hong Liu
Jan 13, 2019·Nature Communications·Viviana BarraDaniele Fachinetti
May 24, 2019·Current Genetics·Prashant K Mishra, Munira A Basrai
Apr 26, 2019·Archives of Pharmacal Research·Ja-Eun Kim
Feb 3, 2020·Cellular and Molecular Life Sciences : CMLS·Charmaine Yan Yu WongKaren Wing Yee Yuen
Aug 28, 2021·International Journal of Molecular Sciences·Shelby L McVeyNatalie J Nannas

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
co-immunoprecipitation
immunoprecipitation
electrophoresis
FCS

Software Mentioned

ImageJ
GraphPad Prism
GraphPad
Zeiss Axiovision

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.