Aurora A inhibition by MNL8054 promotes centriole elongation during Drosophila male meiosis

Cell Cycle
Marco GottardoM G Riparbelli

Abstract

Aurora A kinase plays an important role in several aspects of cell division, including centrosome maturation and separation, a crucial step for the correct organization of the bipolar spindle. Although it has long been showed that this kinase accumulates at the centrosome throughout mitosis its precise contribution to centriole biogenesis and structure has until now not been reported. It is not surprising that so little is known, due to the small size of somatic centrioles, where only dramatic structural changes may be identified by careful electron microscopy analysis. Conversely, centrioles of Drosophila primary spermatocytes increase tenfold in length during the first prophase, thus making any change easily detectable. Therefore, we examined the consequence of the pharmacological inhibition of Aurora A by MLN8054 on centriole biogenesis during early Drosophila gametogenesis. Here, we show that depletion of this kinase results in longer centrioles, mainly during transition from prophase to prometaphase of the first meiosis. We also found abnormal ciliogenesis characterized by irregularly growing axonemal doublets. Our results represent the first documentation of a potential requirement of Aurora A in centriole integrity and e...Continue Reading

References

Aug 1, 1979·Journal of Ultrastructure Research·C L RiederL C Jensen
Mar 1, 1985·The Journal of Cell Biology·G Sluder, C L Rieder
Jun 1, 1982·The Journal of Cell Biology·I A Vorobjev, Chentsov YuS
Aug 27, 2003·The Journal of Cell Biology·Yasuhiko TeradaRyoko Kuriyama
Oct 3, 2003·The Journal of Biological Chemistry·Tomotoshi MarumotoHideyuki Saya
Mar 20, 2004·Developmental Cell·Junmin PanWilliam J Snell
Jun 2, 2005·The Journal of Cell Biology·Lynne M Quarmby, Jeremy D K Parker
Jun 15, 2005·Trends in Cell Biology·Stephen DoxseyKeith Mikule
Sep 28, 2005·The Journal of Cell Biology·Teresa P BarrosJordan W Raff
Jul 25, 2006·Nature·Meng-Fu Bryan Tsou, Tim Stearns
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Mark G ManfrediChristopher F Claiborne
Apr 17, 2007·Current Biology : CB·Christine BlaineauPatrick Bastien
Jul 3, 2007·Cell·Junmin Pan, William Snell
Jul 3, 2007·Cell·Elena N PugachevaErica A Golemis
Aug 24, 2007·Journal of Cell Science·Alexis R Barr, Fanni Gergely
Apr 25, 2008·Molecular Biology of the Cell·Xin ZhangClaire E Walczak
Apr 26, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Nicole Santos, Jeremy F Reiter
Jul 30, 2008·Biochimica Et Biophysica Acta·Gerben Vader, Susanne M A Lens
Aug 30, 2008·The EMBO Journal·Teresa SardonIsabelle Vernos
Mar 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tian PiaoJunmin Pan
Mar 31, 2009·Current Biology : CB·Anne L KnowltonP Todd Stukenberg
Apr 9, 2009·Journal of Cell Science·Chang-Young JangGuowei Fang
Jun 10, 2009·Nature·Neil J GanemDavid Pellman
Sep 18, 2009·Developmental Cell·Meng-Fu Bryan TsouPrasad V Jallepalli
Sep 24, 2009·Environmental and Molecular Mutagenesis·Kara B Lukasiewicz, Wilma L Lingle
Oct 20, 2009·Current Opinion in Cell Biology·Mar CarmenaWilliam C Earnshaw
Feb 11, 2010·Journal of Cell Science·E Scott Seeley, Maxence V Nachury
May 19, 2010·The Journal of Cell Biology·Pierre RoméRégis Giet
Jul 21, 2010·Developmental Cell·Doris KinzelHeiko Lickert
Sep 8, 2010·The EMBO Journal·Hannah MüllerBodo M H Lange
Sep 28, 2010·Current Biology : CB·Juliette Azimzadeh, Wallace F Marshall
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Vladimir JoukovDavid M Livingston
Mar 12, 2011·Nature Cell Biology·Sehyun KimLeonidas Tsiokas
Mar 24, 2011·Biochemical and Biophysical Research Communications·Chang-Young Jang, Guowei Fang
May 4, 2011·The Journal of Cell Biology·Tetsuo Kobayashi, Brian D Dynlacht
May 18, 2011·The Journal of Cell Biology·Won-Jing WangMeng-Fu Bryan Tsou
Aug 5, 2011·Cell Cycle·Sehyun Kim, Leonidas Tsiokas
Dec 21, 2011·The Journal of Cell Biology·Nadia HégaratHelfrid Hochegger
Jan 17, 2012·Current Opinion in Cell Biology·Robert Mahen, Ashok R Venkitaraman

❮ Previous
Next ❯

Citations

Feb 14, 2020·Reproduction in Domestic Animals = Zuchthygiene·Panpan CuiShiqiang Ju
Sep 6, 2019·G3 : Genes - Genomes - Genetics·Boyang LiuJörg Großhans

❮ Previous
Next ❯

Methods Mentioned

BETA
electron microscopy

Software Mentioned

Adobe Photoshop

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.