Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins

Science Signaling
Isha NasaGreg B G Moorhead

Abstract

Protein phosphatase 1 (PP1) is a highly conserved protein phosphatase that performs most of the serine- and threonine-dephosphorylation reactions in eukaryotes and opposes the actions of a diverse set of serine and threonine (Ser-Thr) protein kinases. PP1 gains substrate specificity through binding to a large number (>200) of regulatory proteins that control PP1 localization, activity, and interactions with substrates. PP1 recognizes the well-characterized RVxF binding motif that is present in many of these regulatory proteins, thus generating a multitude of distinct PP1 holoenzymes. We showed that a subset of the RVxF binding motifs, in which x is a phosphorylatable amino acid (RV[S/T]F), was phosphorylated specifically during mitosis and that this phosphorylation event abrogated the interaction of PP1 with the regulatory protein. We determined that this phosphorylation was primarily governed by the mitotic protein kinase Aurora B and that high phosphorylation site stoichiometry of these sites maintained the phosphorylation of PP1 substrates during mitosis by disrupting the assembly of PP1 holoenzymes. We generated an antibody that recognizes the phosphorylated form of the RV[S/T]F motif (RVp[S/T]F) and used it to identify kno...Continue Reading

References

May 1, 1993·Trends in Biochemical Sciences·M J Hubbard, P Cohen
Jan 17, 2003·Molecular Biology of the Cell·Laura Trinkle-MulcahyAngus I Lamond
Mar 27, 2003·The Journal of Biological Chemistry·Paulina WakulaMathieu Bollen
Apr 17, 2003·Journal of Cell Science·Rikke L SteenPhilippe Collas
Jan 13, 2004·Physiological Reviews·Hugo Ceulemans, Mathieu Bollen
May 28, 2004·Nature·Mohammed TerrakRoberto Dominguez
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Nov 19, 2004·Nature·Michael B Kastan, Jiri Bartek
Feb 24, 2006·The Journal of Cell Biology·Laura Trinkle-MulcahyAngus I Lamond
Mar 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marjaana NousiainenRoman Körner
Nov 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Beth A RasalaDouglass J Forbes
Feb 24, 2007·Nature Reviews. Molecular Cell Biology·Greg B G MoorheadAnnegret Ulke-Lemée
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Mark G ManfrediChristopher F Claiborne
Mar 17, 2007·The EMBO Journal·Michela ZuccoloValérie Doye
Jul 20, 2007·The Journal of Biological Chemistry·Thomas D HurleyAnna A DePaoli-Roach
Apr 23, 2008·The Journal of Cell Biology·Michael J EmanueleP Todd Stukenberg
Aug 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Noah DephoureSteven P Gygi
Sep 4, 2008·Science Signaling·Martin Lee MillerRune Linding
Nov 13, 2008·BMC Biochemistry·Greg B G MoorheadAngus I Lamond
Apr 25, 2009·Chemistry & Biology·Annick HendrickxMathieu Bollen
Dec 17, 2009·Methods in Molecular Biology·Joshua E Elias, Steven P Gygi
Apr 20, 2010·Trends in Biochemical Sciences·Mathieu BollenMonique Beullens
Sep 24, 2010·Molecular & Cellular Proteomics : MCP·Anna SantamariaErich A Nigg
Nov 4, 2010·BMC Cell Biology·Julie HigginsJacquelyn Bond
Nov 26, 2010·Trends in Cell Biology·Michael A Lampson, Iain M Cheeseman
Dec 7, 2010·Nature Methods·Hyungwon ChoiAlexey I Nesvizhskii
May 20, 2011·Nature·Björn SchwanhäusserMatthias Selbach
Jun 7, 2011·Current Biology : CB·Jessica S RosenbergHironori Funabiki
Jun 15, 2011·Developmental Cell·John C MeadowsJonathan B A Millar
Jun 29, 2011·Journal of Cell Science·Francis A BarrUlrike Gruneberg
Jul 14, 2011·Proteomics·Benoît ValotMichel Zivy
Aug 9, 2011·Developmental Cell·Paola VagnarelliWilliam C Earnshaw
Nov 15, 2011·Current Biology : CB·Bart LesageMathieu Bollen
Mar 1, 2012·The FEBS Journal·Ewald HeroesMathieu Bollen

❮ Previous
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Citations

Nov 30, 2018·Molecular Biology of the Cell·Jovana DereticJulie P I Welburn
Feb 10, 2019·Biomolecules·Margarida Moura, Carlos Conde
Jan 30, 2020·Cell Cycle·M Lienhard SchmitzMarkus Seibert
Jun 6, 2020·Essays in Biochemistry·Dimitriya H Garvanska, Jakob Nilsson
Aug 31, 2019·FEBS Letters·Daniel HaywardUlrike Gruneberg
Aug 16, 2018·Science Signaling·Vladimir Joukov, Arcangela De Nicolo
Feb 16, 2019·The Journal of Cell Biology·Markus SeibertM Lienhard Schmitz
Nov 18, 2018·The Journal of Cell Biology·Jakob Nilsson
Jun 19, 2019·Proceedings of the National Academy of Sciences of the United States of America·Tatiana N MoiseevaChristopher J Bakkenist
Sep 18, 2020·The Journal of Biological Chemistry·Guangwei XinChuanmao Zhang
Oct 31, 2020·Biochemical Society Transactions·Sarah Lemaire, Mathieu Bollen
Aug 14, 2018·Structure·Rakhi BajajRebecca Page
Apr 4, 2021·Seminars in Cell & Developmental Biology·Paola Vagnarelli
May 25, 2021·The FEBS Journal·Xinyu CaoMathieu Bollen
Jul 3, 2021·Seminars in Cell & Developmental Biology·Pablo Lara-GonzalezArshad Desai
Sep 2, 2021·Nucleic Acids Research·Diletta CiardoKathrin Marheineke

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