Aurora B prevents chromosome arm separation defects by promoting telomere dispersion and disjunction

The Journal of Cell Biology
Céline ReyesSylvie Tournier

Abstract

The segregation of centromeres and telomeres at mitosis is coordinated at multiple levels to prevent the formation of aneuploid cells, a phenotype frequently observed in cancer. Mitotic instability arises from chromosome segregation defects, giving rise to chromatin bridges at anaphase. Most of these defects are corrected before anaphase onset by a mechanism involving Aurora B kinase, a key regulator of mitosis in a wide range of organisms. Here, we describe a new role for Aurora B in telomere dispersion and disjunction during fission yeast mitosis. Telomere dispersion initiates in metaphase, whereas disjunction takes place in anaphase. Dispersion is promoted by the dissociation of Swi6/HP1 and cohesin Rad21 from telomeres, whereas disjunction occurs at anaphase after the phosphorylation of condensin subunit Cnd2. Strikingly, we demonstrate that deletion of Ccq1, a telomeric shelterin component, rescued cell death after Aurora inhibition by promoting the loading of condensin on chromosome arms. Our findings reveal an essential role for telomeres in chromosome arm segregation.

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Citations

Jun 17, 2016·Molecular & Cellular Oncology·Yannick GachetSylvie Tournier
Sep 12, 2015·Molecular Biology of the Cell·Bettina A MoserToru M Nakamura
Feb 27, 2020·Molecular Biology of the Cell·Julien BerthezeneSylvie Tournier
May 15, 2018·The Journal of Pathology·Vassilis G GorgoulisIoannis P Trougakos
Sep 27, 2018·Cell Division·Estelle WillemsBernard Rogister
Sep 12, 2015·Journal of Cell Science·Hadrien MaryYannick Gachet

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Methods Mentioned

BETA
immunoprecipitation
dissection
PCR

Software Mentioned

ImageJ
WaveMetrics
Adobe Photoshop
MetaMorph
IGOR

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