Aurora kinase A (AURKA) interaction with Wnt and Ras-MAPK signalling pathways in colorectal cancer.

Scientific Reports
Annika JacobsenK Anton Feenstra

Abstract

Hyperactivation of Wnt and Ras-MAPK signalling are common events in development of colorectal adenomas. Further progression from adenoma-to-carcinoma is frequently associated with 20q gain and overexpression of Aurora kinase A (AURKA). Interestingly, AURKA has been shown to further enhance Wnt and Ras-MAPK signalling. However, the molecular details of these interactions in driving colorectal carcinogenesis remain poorly understood. Here we first performed differential expression analysis (DEA) of AURKA knockdown in two colorectal cancer (CRC) cell lines with 20q gain and AURKA overexpression. Next, using an exact algorithm, Heinz, we computed the largest connected protein-protein interaction (PPI) network module of significantly deregulated genes in the two CRC cell lines. The DEA and the Heinz analyses suggest 20 Wnt and Ras-MAPK signalling genes being deregulated by AURKA, whereof β-catenin and KRAS occurred in both cell lines. Finally, shortest path analysis over the PPI network revealed eight 'connecting genes' between AURKA and these Wnt and Ras-MAPK signalling genes, of which UBE2D1, DICER1, CDK6 and RACGAP1 occurred in both cell lines. This study, first, confirms that AURKA influences deregulation of Wnt and Ras-MAPK sig...Continue Reading

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Citations

Jul 10, 2019·International Journal of Colorectal Disease·Vittorio BranchiSebastian Schölch
Nov 30, 2018·Journal of Experimental & Clinical Cancer Research : CR·Chuanchuan ZhouShuhan Sun
Dec 5, 2019·World Journal of Clinical Cases·Hussein SabitHuseyin Tombuloglu
Aug 8, 2021·Cancers·Anxo Rio-VilariñoArancha Cebrián
Nov 1, 2021·Liver International : Official Journal of the International Association for the Study of the Liver·Yanze YinQiang Xia
Mar 13, 2020·Molecular Therapy. Nucleic Acids·Jaitip TipaneeThierry VandenDriessche

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Datasets Mentioned

BETA
GSE108320

Methods Mentioned

BETA
chromosomal aberrations
RNA-seq
transfection

Software Mentioned

VENN
Limma
Ensembl
combat
NetworkX
Bioconductor
SVA
Gene Ontology enRIchment anaLysis and visuaLizAtion
R

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