Authentic role of ATP signaling in micturition reflex

Scientific Reports
Kentaro TakezawaShoichi Shimada

Abstract

Adenosine triphosphate (ATP) is a signaling molecule that regulates cellular processes. Based on previous studies of bladder function over the past decade, bladder ATP signaling was thought to have an essential role in the normal micturition reflex. In this study, we performed detailed analyses of bladder function in purinergic receptor-deficient mice using the automated voided stain on paper method and video-urodynamics. Unexpectedly, a lack of P2X2 or P2X3 receptors did not affect bladder function under normal physiological conditions, indicating that bladder ATP signaling is not essential for normal micturition reflex. In contrast, we found that lipopolysaccharide (LPS) induced markedly high levels of ATP release from the urothelium. In addition, LPS-induced rapid bladder hyperactivity was attenuated in P2X2(-/-) and P2X3(-/-) mice. Contrary to the previous interpretation, our present findings indicate that bladder ATP signaling has a fundamental role in the micturition reflex, especially in bladder dysfunction, under pathological conditions. Therefore, the bladder ATP signaling pathway might be a highly promising therapeutic target for functional bladder disorders. This study newly defines an authentic role for bladder ATP ...Continue Reading

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Citations

Aug 20, 2016·Neurourology and Urodynamics·Kentaro TakezawaShoichi Shimada
Feb 2, 2018·Scientific Reports·Atsushi SengikuHiromitsu Negoro
Mar 20, 2020·Physiological Reviews·Marianela G DalghiGerard Apodaca
Oct 16, 2020·Nature·Kara L MarshallArdem Patapoutian
Oct 23, 2020·Lower Urinary Tract Symptoms·Kareman EljamalToshiyuki Sasaguri
Jul 31, 2021·Autonomic Neuroscience : Basic & Clinical·Christopher H Fry, Karen D McCloskey

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Methods Mentioned

BETA
ion-exchange chromatography
PCR

Software Mentioned

JMP
Image J
aVSOP

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