Authentication of food allergen quality by physicochemical and immunological methods.

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
Ana I SanchoStefan Vieths

Abstract

Purified allergens are required to detect cross-contamination with other allergenic foods and to understand allergen interaction with other components of the food matrix. Pure allergens are also used for the diagnosis and treatment of food allergies. For example, serological methods are being developed to improve the quality of diagnosis, and to reduce the need for food challenge tests. In addition, recombinant allergens are being evaluated as candidate vaccines for safe and efficacious specific immunotherapy. Pure allergens are indispensable as reference materials for the calibration and standardization of methods between different laboratories and operators for risk assessment in the food industry. Therefore, there is a need for well-defined purified food allergens. In this context, a panel of 46 food allergens from plant and animal sources has been purified, from either the food sources or as recombinant forms, within the EU-funded EuroPrevall project. These allergens have been characterized by a battery of diagnostic tests demonstrating that they constitute an authentic, well-defined library of comparable quality. The review summarizes the applications, potentials and limitations of key techniques used for the characterizat...Continue Reading

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Citations

Apr 16, 2011·Clinical Reviews in Allergy & Immunology·Carlo Selmi
Jul 7, 2012·PloS One·Stefano AlessandriKarin Hoffmann-Sommergruber
Apr 17, 2014·Clinical and Translational Allergy·Lars K PoulsenRonald van Ree
Jan 17, 2012·Immunology and Allergy Clinics of North America·Jean-Christoph Caubet, Hugh A Sampson
Dec 14, 2011·Molecular Nutrition & Food Research·Ivana AleksicMarija Gavrovic-Jankulovic
Jun 11, 2014·Journal of the Science of Food and Agriculture·Dana FlodrováMarkéta Laštovičková
Aug 4, 2015·Current Allergy and Asthma Reports·Karin Hoffmann-SommergruberMerima Bublin
Jul 9, 2011·Journal of Chromatography. a·Gianluca PicarielloPasquale Ferranti

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